Abstract
3D-printing is a novel method for the fabrication of layer-by-layer three-dimensional features with high precision. Digital Light Processing (DLP) 3D printing is the most suited technique for the fabrication of complex microfluidic devices with a large number of interconnected microchannels, as encountered in spatial multi-dimensional liquid chromatography (MD-LC). This novel separation concept requires the construction of a device in which analytes are separated by their position in a three-dimensional separation space.
In this research, several aspects of DLP 3D-printing were investigated and optimized, including the optimization of the printing parameters, microchannel design, and postfabrication process. A comprehensive-experimental study about effects of exposure time and layer thickness on microchannel geometry and surface waviness was conducted. A spatial 2D-LC chip was designed and prototyped targeting isoelectric focusing followed by size-exclusion chromatography (IEF x SEC). A novel methodology for localized synthesizing
UV-photoinitiated polymer monolithic frits in-situ in microdevices created from a UV absorber 3D-printing resin was developed. Furthermore, the parallel channel structure was packed with 5 μm SEC particles and employed for the separation of intact proteins. The pressure resistance of the 3D-printed chips was determined to be around 230 bar in presence of organic solvents.
In this research, several aspects of DLP 3D-printing were investigated and optimized, including the optimization of the printing parameters, microchannel design, and postfabrication process. A comprehensive-experimental study about effects of exposure time and layer thickness on microchannel geometry and surface waviness was conducted. A spatial 2D-LC chip was designed and prototyped targeting isoelectric focusing followed by size-exclusion chromatography (IEF x SEC). A novel methodology for localized synthesizing
UV-photoinitiated polymer monolithic frits in-situ in microdevices created from a UV absorber 3D-printing resin was developed. Furthermore, the parallel channel structure was packed with 5 μm SEC particles and employed for the separation of intact proteins. The pressure resistance of the 3D-printed chips was determined to be around 230 bar in presence of organic solvents.
| Original language | English |
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| Publication status | Published - Sep 2022 |
| Event | 33rd International Symposium on Chromatography – ISC 2022 - Budapest, Hungary Duration: 18 Sep 2022 → 22 Sep 2022 |
Conference
| Conference | 33rd International Symposium on Chromatography – ISC 2022 |
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| Country | Hungary |
| City | Budapest |
| Period | 18/09/22 → 22/09/22 |
Keywords
- 3D printing
- multi-dimensional LC