Optimization of the 3D-Printing Fabrication Processes for Prototyping Microfluidic Devices for Spatial Multi-dimensional Liquid Chromatography

Research output: Unpublished contribution to conferencePoster

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3D-printing is a novel method for the fabrication of layer-by-layer three-dimensional features with high precision. Digital Light Processing (DLP) 3D printing is the most suited technique for the fabrication of complex microfluidic devices with a large number of interconnected microchannels, as encountered in spatial multi-dimensional liquid chromatography (MD-LC). This novel separation concept requires the construction of a device in which analytes are separated by their position in a three-dimensional separation space.

In this research, several aspects of DLP 3D-printing were investigated and optimized, including the optimization of the printing parameters, microchannel design, and postfabrication process. A comprehensive-experimental study about effects of exposure time and layer thickness on microchannel geometry and surface waviness was conducted. A spatial 2D-LC chip was designed and prototyped targeting isoelectric focusing followed by size-exclusion chromatography (IEF x SEC). A novel methodology for localized synthesizing
UV-photoinitiated polymer monolithic frits in-situ in microdevices created from a UV absorber 3D-printing resin was developed. Furthermore, the parallel channel structure was packed with 5 μm SEC particles and employed for the separation of intact proteins. The pressure resistance of the 3D-printed chips was determined to be around 230 bar in presence of organic solvents.
Original languageEnglish
Publication statusPublished - Sep 2022
Event33rd International Symposium on Chromatography – ISC 2022 - Budapest, Hungary
Duration: 18 Sep 202222 Sep 2022


Conference33rd International Symposium on Chromatography – ISC 2022


  • 3D printing
  • multi-dimensional LC

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