Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography

Theodora Adamopoulou; Sander Deridder; Tijmen S. Bos; Suhas Nawada; Gert Desmet; Peter J. Schoenmakers

doi:10.1016/j.chroma.2019.460665

Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography

Theodora Adamopoulou, Sander Deridder, Tijmen S. Bos, Suhas Nawada, Gert Desmet, Peter J. Schoenmakers

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Abstract

In spatial multi-dimensional liquid chromatography (LC) devices the flow of each dimension has to remain in the corresponding region, otherwise the separation efficiency is undermined. Adequate flow-confinement measures are necessary. Here, the use of permeability differences across different compartments of spatial two-dimensional (2D) and three-dimensional (3D) LC devices as a method to guide fluid flow and reduce analyte loss during the first, second- and third-dimension development was investigated with computational fluid dynamics (CFD) simulations. In case of 2DLC devices, it was shown that porous barriers with a permeability on the order of 10(-12) m(2) suffice to keep the total sample spillage from an open D-1 channel under 1%. In case of 3DLC devices, it was shown that flow confinement could be achieved using an open D-1 channel in combination with a highly-permeable monolith (permeability on the order of 10(-12) m(2)) in the second-dimension (D-2) and a less permeable packing with a permeability on the order of 10(-15) m(2) (e.g. 1 mu m particles) in the third-dimension (D-3). Additionally, the impact of the D-3 flow-distributor has been studied and a novel design, capable of limiting the spillage to the other dimensions to the absolute minimum, is proposed. (C) 2019 Elsevier B.V. All rights reserved.

Original language	English
Article number	460665
Journal	Journal of Chromatography A
Volume	1612
DOIs	https://doi.org/10.1016/j.chroma.2019.460665
Publication status	Published - 8 Feb 2020

Bibliographical note

Funding Information:
The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC) , Project 694151 . The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation – Flanders (FWO-Vlaanderen) .

Funding Information:
The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC), Project 694151. The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation ? Flanders (FWO-Vlaanderen).

Publisher Copyright:
© 2019 Elsevier B.V.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

Multi-dimensional LC
Spatial chromatography
Computational fluid dynamics
Flow-control
Flow-confinement

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Manuscript_Adamopoulou_JCA2020_Optimizing designAccepted author manuscript, 2.06 MBLicence: CC BY-NC-ND

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title = "Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography",

abstract = "In spatial multi-dimensional liquid chromatography (LC) devices the flow of each dimension has to remain in the corresponding region, otherwise the separation efficiency is undermined. Adequate flow-confinement measures are necessary. Here, the use of permeability differences across different compartments of spatial two-dimensional (2D) and three-dimensional (3D) LC devices as a method to guide fluid flow and reduce analyte loss during the first, second- and third-dimension development was investigated with computational fluid dynamics (CFD) simulations. In case of 2DLC devices, it was shown that porous barriers with a permeability on the order of 10(-12) m(2) suffice to keep the total sample spillage from an open D-1 channel under 1%. In case of 3DLC devices, it was shown that flow confinement could be achieved using an open D-1 channel in combination with a highly-permeable monolith (permeability on the order of 10(-12) m(2)) in the second-dimension (D-2) and a less permeable packing with a permeability on the order of 10(-15) m(2) (e.g. 1 mu m particles) in the third-dimension (D-3). Additionally, the impact of the D-3 flow-distributor has been studied and a novel design, capable of limiting the spillage to the other dimensions to the absolute minimum, is proposed. (C) 2019 Elsevier B.V. All rights reserved.",

keywords = "Multi-dimensional LC, Spatial chromatography, Computational fluid dynamics, Flow-control, Flow-confinement",

author = "Theodora Adamopoulou and Sander Deridder and Bos, {Tijmen S.} and Suhas Nawada and Gert Desmet and Schoenmakers, {Peter J.}",

note = "Funding Information: The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC) , Project 694151 . The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation – Flanders (FWO-Vlaanderen) . Funding Information: The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC), Project 694151. The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation ? Flanders (FWO-Vlaanderen). Publisher Copyright: {\textcopyright} 2019 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = feb,

day = "8",

doi = "10.1016/j.chroma.2019.460665",

language = "English",

volume = "1612",

journal = "Journal of Chromatography A",

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T1 - Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography

AU - Adamopoulou, Theodora

AU - Deridder, Sander

AU - Bos, Tijmen S.

AU - Nawada, Suhas

AU - Desmet, Gert

AU - Schoenmakers, Peter J.

N1 - Funding Information: The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC) , Project 694151 . The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation – Flanders (FWO-Vlaanderen) . Funding Information: The STAMP project is funded under Horizon 2020-Excellent Science-European Research Council (ERC), Project 694151. The sole responsibility of this publication lies with the authors. The European Union is not responsible for any use that may be made of the information contained therein. Sander Deridder gratefully acknowledges a research grant from the Research Foundation ? Flanders (FWO-Vlaanderen). Publisher Copyright: © 2019 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/2/8

Y1 - 2020/2/8

N2 - In spatial multi-dimensional liquid chromatography (LC) devices the flow of each dimension has to remain in the corresponding region, otherwise the separation efficiency is undermined. Adequate flow-confinement measures are necessary. Here, the use of permeability differences across different compartments of spatial two-dimensional (2D) and three-dimensional (3D) LC devices as a method to guide fluid flow and reduce analyte loss during the first, second- and third-dimension development was investigated with computational fluid dynamics (CFD) simulations. In case of 2DLC devices, it was shown that porous barriers with a permeability on the order of 10(-12) m(2) suffice to keep the total sample spillage from an open D-1 channel under 1%. In case of 3DLC devices, it was shown that flow confinement could be achieved using an open D-1 channel in combination with a highly-permeable monolith (permeability on the order of 10(-12) m(2)) in the second-dimension (D-2) and a less permeable packing with a permeability on the order of 10(-15) m(2) (e.g. 1 mu m particles) in the third-dimension (D-3). Additionally, the impact of the D-3 flow-distributor has been studied and a novel design, capable of limiting the spillage to the other dimensions to the absolute minimum, is proposed. (C) 2019 Elsevier B.V. All rights reserved.

AB - In spatial multi-dimensional liquid chromatography (LC) devices the flow of each dimension has to remain in the corresponding region, otherwise the separation efficiency is undermined. Adequate flow-confinement measures are necessary. Here, the use of permeability differences across different compartments of spatial two-dimensional (2D) and three-dimensional (3D) LC devices as a method to guide fluid flow and reduce analyte loss during the first, second- and third-dimension development was investigated with computational fluid dynamics (CFD) simulations. In case of 2DLC devices, it was shown that porous barriers with a permeability on the order of 10(-12) m(2) suffice to keep the total sample spillage from an open D-1 channel under 1%. In case of 3DLC devices, it was shown that flow confinement could be achieved using an open D-1 channel in combination with a highly-permeable monolith (permeability on the order of 10(-12) m(2)) in the second-dimension (D-2) and a less permeable packing with a permeability on the order of 10(-15) m(2) (e.g. 1 mu m particles) in the third-dimension (D-3). Additionally, the impact of the D-3 flow-distributor has been studied and a novel design, capable of limiting the spillage to the other dimensions to the absolute minimum, is proposed. (C) 2019 Elsevier B.V. All rights reserved.

KW - Multi-dimensional LC

KW - Spatial chromatography

KW - Computational fluid dynamics

KW - Flow-control

KW - Flow-confinement

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U2 - 10.1016/j.chroma.2019.460665

DO - 10.1016/j.chroma.2019.460665

M3 - Article

SN - 0021-9673

VL - 1612

JO - Journal of Chromatography A

JF - Journal of Chromatography A

M1 - 460665

ER -

Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography

Abstract

Bibliographical note

Keywords

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SRP6: Strategic Research Programme: Exploiting the Advantages of Order and Geometrical Structure for a Greener Chemistry

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Optimizing design and employing permeability differences to achieve flow confinement in devices for spatial multidimensional liquid chromatography

Abstract

Bibliographical note

Keywords

Access to Document

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SRP6: Strategic Research Programme: Exploiting the Advantages of Order and Geometrical Structure for a Greener Chemistry

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