ORAL PRES.: Chromatographic herbal fingerprints: Development, Validation and Data Handling

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

The use of herbal medicines is gaining importance, even in Western medicine. In any herbal extract/medicine, however, there are many unknown components, which are often only present in low amounts. Identifying only one or some compounds hardly describes the complex extracts, and will thus not be reliable enough for their quality control. A possible solution is the use of ‘fingerprint technology’, which can, for example, be performed by means of High-Performance Liquid Chromatography (HPLC). This technology has been approved by the World Health Organization, the Chinese State Food and Drug Administration, the USA Food and Drug Administration, and the European Agency for the Evaluation of Medicinal Products. The idea is to develop a complete chromatographic pattern of the herbal extract, i.e. a chromatographic fingerprint, in which usually as many compounds as possible are separated.
When using the fingerprint technology, three main steps are considered, i.e. the fingerprint development, its validation, and the extraction of information from the fingerprint. Fingerprint development can be further divided into a sample preparation step and an actual fingerprint development step (i.e. determining the optimal analytical conditions) [1]. To validate the entire fingerprints, a set-up can be used to determine simultaneously the repeatability and the time-different intermediate precision of e.g. the retention time and peak area/height of given peaks in the fingerprint [2].
Considering the data handling, depending on the goal of the study, (1) data pretreatment, (2) identification and quality control, (3) exploratory analysis, (4) pattern recognition, (5) curve resolution, and/or (6) multivariate calibration techniques can be used [3-8]. Different aspects of data handling of chromatographic herbal fingerprints will be discussed and illustrated with some examples.
Original languageEnglish
Title of host publicationJoint meeting of the GP-TCM Research Association, the TCM Chemistry Specialty Committee and the TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies; 13th-15th July 2015 - Mons - Belgium
Publication statusPublished - 2015
EventJoint meeting of the GP-TCM Research Association, the TCM Chemistry Specialty Committee and the TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies; 13th-15th July 2015 - Mons - Belgium - Mons, Belgium
Duration: 13 Jul 201515 Jul 2015

Conference

ConferenceJoint meeting of the GP-TCM Research Association, the TCM Chemistry Specialty Committee and the TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies; 13th-15th July 2015 - Mons - Belgium
CountryBelgium
CityMons
Period13/07/1515/07/15

Fingerprint Dive into the research topics of 'ORAL PRES.: Chromatographic herbal fingerprints: Development, Validation and Data Handling'. Together they form a unique fingerprint.

Cite this