Outcomes of Human Leukocyte Antigen-Matched Allogeneic Cultivated Limbal Epithelial Transplantation in Aniridia-Associated Keratopathy-A Single-Center Retrospective Analysis

Joséphine Behaegel, Marie-José Tassignon, Neil Lagali, Alejandra Consejo, Carina Koppen, Sorcha Ní Dhubhghaill

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

PURPOSE: To assess the efficacy and safety of human leukocyte antigen-matched allogeneic cultivated limbal epithelial stem cell grafts in the treatment of aniridia-associated keratopathy (AAK).

METHODS: Six eyes of 6 patients with severe AAK received an allogeneic stem cell graft between January 2010 and March 2017. Anatomical and functional results were assessed at 6 months, 1 year, 2 years, and the final follow-up visit available. Safety analysis was performed by considering all perioperative and postoperative adverse events and additional surgeries required during the follow-up period.

RESULTS: The mean follow-up was 53.6 months (range 24-104 months). In most patients (80%), there was an early improvement of the keratopathy postoperatively, which slowly regressed during longer follow-up. At the final follow-up, 4 of the eyes were graded as failure and 1 eye was graded as partial success. Grading the sixth eye was not possible because of an adverse event. None of the patients maintained a total anatomical success in the long-term. Only 1 patient maintained a modest improvement in best-corrected visual acuity from hand motion to counting fingers. Four serious adverse events were recorded in 2 patients.

CONCLUSIONS: Severe AAK remains a challenging condition to manage. Transplantation of allogenic ex vivo cultivated limbal stem cells may provide a temporary improvement in ocular surface stability, but anatomical and functional results are poor in the long-term. The eyes are prone to adverse events, and any surgical treatment should take this into consideration.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalCornea
Volume41
Issue number1
DOIs
Publication statusPublished - 1 Jan 2022

Keywords

  • Adult
  • Aged
  • Aniridia/complications
  • Cells, Cultured
  • Corneal Diseases/diagnosis
  • Epithelium, Corneal/cytology
  • Female
  • Follow-Up Studies
  • Graft Survival
  • HLA Antigens/immunology
  • Humans
  • Limbus Corneae/cytology
  • Male
  • Middle Aged
  • Retrospective Studies
  • Stem Cell Transplantation/methods
  • Stem Cells/cytology
  • Time Factors
  • Transplantation, Autologous
  • Visual Acuity
  • Young Adult

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