Performance of laterally elongated pillar array columns in capillary electrochromatography mode

Martyna Baca; Agata Kryj; Elahe Naghdi; Pierre Gelin; Sertan Sukas; Priya Laha; Herman Terryn; Heidi Ottevaere; Wim De Malsche

doi:10.1002/elps.202000001

Performance of laterally elongated pillar array columns in capillary electrochromatography mode

Martyna Baca, Agata Kryj, Elahe Naghdi, Pierre Gelin, Sertan Sukas, Priya Laha, Herman Terryn, Heidi Ottevaere, Wim De Malsche

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

163 Downloads (Pure)

Abstract

In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.

Original language	English
Pages (from-to)	1287-1295
Number of pages	9
Journal	Electrophoresis
Volume	41
Issue number	15
DOIs	https://doi.org/10.1002/elps.202000001 https://doi.org/10.1002/elps.202000001
Publication status	Published - Aug 2020

Bibliographical note

Funding Information:
. W.D.M. and M.B. greatly acknowledge the European Research Council for support through an ERC Starting Grant (number 679033EVODIS ERC‐2015‐STG)

Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Copyright:
Copyright 2023 Elsevier B.V., All rights reserved.

Keywords

CEC
microfluidic chips
pillar array columns

Access to Document

Baca et al, 20-04-2020_trackchanges
The author(s) opt to apply XI.196. § 2/1 book 1 “intellectuele eigendommen” of the Belgian Federal Code of Economic Law in order to obey funder requirements.
Accepted author manuscript, 542 KB

Cite this

@article{4e32dfb278e24d85bc1f3a7c254ac569,

title = "Performance of laterally elongated pillar array columns in capillary electrochromatography mode",

abstract = "In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.",

keywords = "CEC, microfluidic chips, pillar array columns",

author = "Martyna Baca and Agata Kryj and Elahe Naghdi and Pierre Gelin and Sertan Sukas and Priya Laha and Herman Terryn and Heidi Ottevaere and Malsche, {Wim De}",

note = "Funding Information: . W.D.M. and M.B. greatly acknowledge the European Research Council for support through an ERC Starting Grant (number 679033EVODIS ERC‐2015‐STG) Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2023 Elsevier B.V., All rights reserved.",

year = "2020",

month = aug,

doi = "10.1002/elps.202000001",

language = "English",

volume = "41",

pages = "1287--1295",

journal = "Electrophoresis",

issn = "0173-0835",

publisher = "Wiley-VCH Verlag",

number = "15",

}

TY - JOUR

T1 - Performance of laterally elongated pillar array columns in capillary electrochromatography mode

AU - Baca, Martyna

AU - Kryj, Agata

AU - Naghdi, Elahe

AU - Gelin, Pierre

AU - Sukas, Sertan

AU - Laha, Priya

AU - Terryn, Herman

AU - Ottevaere, Heidi

AU - Malsche, Wim De

N1 - Funding Information: . W.D.M. and M.B. greatly acknowledge the European Research Council for support through an ERC Starting Grant (number 679033EVODIS ERC‐2015‐STG) Publisher Copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2023 Elsevier B.V., All rights reserved.

PY - 2020/8

Y1 - 2020/8

N2 - In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.

AB - In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.

KW - CEC

KW - microfluidic chips

KW - pillar array columns

UR - http://www.scopus.com/inward/record.url?scp=85084842428&partnerID=8YFLogxK

U2 - 10.1002/elps.202000001

DO - 10.1002/elps.202000001

M3 - Article

AN - SCOPUS:85084842428

VL - 41

SP - 1287

EP - 1295

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 15

ER -

Performance of laterally elongated pillar array columns in capillary electrochromatography mode

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

SRP51: SRP-Onderzoekszwaartepunt: Optical and microfluidic tools to unravel the dynamics of bio-condensates

Cite this

Performance of laterally elongated pillar array columns in capillary electrochromatography mode

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Projects

SRP51: SRP-Onderzoekszwaartepunt: Optical and microfluidic tools to unravel the dynamics of bio-condensates

Cite this