Polymer-based microfluidic devices for spatial two- and three-dimensional liquid chromatography

Research output: Unpublished contribution to conferenceUnpublished paper

Abstract

The diversification of the cellular proteome is the result of protein synthesis by translation of mRNA and the formation of protein isoforms. Using current liquid-chromatographic mass-spectrometry (LC-MS) techniques, including two-dimensional LC-MS strategies, it is only possible to assess the top 1000 proteins due to limited resolving power. In order to successfully tackle these complex separations, the development of novel multi-dimensional separation technology is required, addressing both the bottlenecks of efficiency and analysis time.
In spatial chromatography, components are separated in the space domain with each peak being characterized by its coordinates in a plane or three-dimensional separation body. Due to parallel developments in the second and third dimension, analysis time is greatly reduced compared to a coupled-column multi-dimensional LC approach. Spatial three-dimensional LC has the potential to offer unrivaled peak capacity and peak-production rate.
The design of microfluidic devices for comprehensive spatial multi-dimensional chromatography will be discussed. The device for two-dimensional chromatography features a first-dimension (1D) separation channel and 21 parallel second-dimension (2D) separation channels, orientated perpendicular to the 1D channel. Compartmentalization of 1D and 2D flow was achieved integrating a physical barrier that allowed for a preferential mobile-phase flow path in the 1D channel, with minimal dispersion into the flow distributor and 2D separation channels. Once the 1D separation is completed, a radially-interconnected flow distributor of diamond-shaped pillars introduces the mobile phase for the 2D separation. Monolithic stationary phase were created in-situ at the desired location via a UV-initiated polymerization reaction and employing photomasks.
Finally, a prototype 3D spatial chip composed of three modules will be demonstrated. The top module is composed of a ramified 3D flow distributor consisting of eight generations of T-bifurcations resulting in 256 outlets. The middle module consists of a modified two-dimensional separation microfluidic device. The bottom module consists of a 256 parallel 3D channels, allowing for the final 3D separation.
Original languageEnglish
Publication statusPublished - 28 Jan 2014
Event13th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analysers, HTC-13 & HTSP-3 - Bruges, Belgium
Duration: 29 Jan 201431 Jan 2014

Conference

Conference13th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analysers, HTC-13 & HTSP-3
CountryBelgium
CityBruges
Period29/01/1431/01/14

Keywords

  • spatial chromatography
  • flow distributor

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