POSTER: Determination of optimal extraction conditions for Pistacia atlantica leaves using response surface methodology.

Ziyad Ben Ahmed, M. Yousfi, Johan Viaene, B. Dejaegher, Debby Mangelings, Kristiaan Demeyer, Yvan Vander Heyden

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Abstract

Response surface methodology (RSM) in combination with Box-Behnken experimental design was performed in this study to optimize the extraction parameters for assessing maximum yield of phenolic antioxidant compounds from male and female leaves of Pistacia atlantica collected in two growing regions i.e Laghouat and Ain oussera.

The aqueous extraction of phenolic compounds from dried powder of P. atlantica leaves were optimized by using the three independent variables, namely extraction time (h), liquid-to-solid ratio (ml/g dry leaf weight) and extraction temperature (°C) were selected after factorial screening. A Box-Behnken design consisting of 15 experimental runs with three replicates at the centre point was then applied and a second-order polynomial model was used to describe the experimental data.

The Box–Behnken design showed that polynomial regression models were in good agreement with the experimental results with the correlation coefficients varied between 0.90 and 0.94 Based on the proposed models, the liquid-to-solid ratio had the most critical role in the extraction of phenolic compounds from both genders of P. atlantica leaves followed by extraction temperature and extraction time. RSM was shown that the both genders of P. atlantica leaves had a similar behaviour in the extraction of TPC. The optimum extraction conditions were found 55°C extraction temperature, 100:2 ml/g liquid-to-solid ratio and 72 hours extraction time. Under the optimized conditions, the content of phenolic compounds varied from 256 to 306.56 mg GAE/ g DW.

The results of this study support the potential of using P. atlantica leaves as a source of natural phenolic antioxidants and give useful directions on how to improve recovery by proper selection of extraction conditions.
Original languageEnglish
Title of host publicationIVth PhD day @ Jette, Vrije Universiteit Brussel, Belgium, 31 March 2015
Publication statusPublished - 2015
EventIVth PhD day @ Jette, Vrije Universiteit Brussel, Belgium, 31 March 2015 - , Belgium
Duration: 31 Mar 201531 Mar 2015

Conference

ConferenceIVth PhD day @ Jette, Vrije Universiteit Brussel, Belgium, 31 March 2015
CountryBelgium
Period31/03/1531/03/15

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