Preimplantation genetic diagnosis (PGD) for Huntington's disease: the experience of three European centres.

M.c. Van Rij, Marjan De Rademaeker, C; Moutou, J. Dreesen, Martine De Rycke, Ingeborg Liebaers, J.p. Geraedts, C.e.m. De Die-Smulders, S. Viville, Working Group Brumastra Pgd

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This study provides an overview of 13 years of experience of preimplantation genetic diagnosis (PGD) for Huntington's disease (HD) at three European PGD centres in Brussels, Maastricht and Strasbourg. Information on all 331 PGD intakes for HD, couples' reproductive history, PGD approach, treatment cycles and outcomes between 1995 and 2008 were collected prospectively. Of 331 couples for intake, 68% requested direct testing and 32% exclusion testing (with a preponderance of French couples). At the time of PGD intake, 39% of women had experienced one or more pregnancies. A history of pregnancy termination after prenatal diagnosis was observed more frequently in the direct testing group (25%) than in the exclusion group (10%; P=0.0027). PGD workup was based on two approaches: (1) direct testing of the CAG-triplet repeat and (2) linkage analysis using intragenic or flanking microsatellite markers of the HTT gene. In total, 257 couples had started workup and 174 couples (70% direct testing, 30% exclusion testing) completed at least one PGD cycle. In total, 389 cycles continued to oocyte retrieval (OR). The delivery rates per OR were 19.8%, and per embryo transfer 24.8%, resulting in 77 deliveries and the birth of 90 children. We conclude that PGD is a valuable and safe reproductive option for HD carriers and couples at risk of transmitting HD.
Original languageEnglish
Pages (from-to)368-375
Number of pages8
JournalEur J Hum Genet
Volume20
Issue numberApril
Publication statusPublished - 2012

Keywords

  • Adult
  • Embryo transfer
  • Europe
  • Female
  • Genetic linkage
  • Humans
  • Huntington Disease
  • Pregnancy
  • Pregnancy Complications
  • Preimplantation Diagnosis
  • PDG

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