Abstract
Background
Hospital-acquired infections, caused by multi-drug resistant, Verona Integron-encoded Metallo-β-lactamase (VIM)-producing P. aeruginosa, are a major problem in intensive care units (ICU). Often, these VIM-producing P. aeruginosa strains are present in the sink drains of the ICU and are nearly impossible to eradicate. Recent studies suggest that certain other microorganisms present in these sink drains could inhibit the growth of VIM-producing P. aeruginosa. In our study set-up, we apply a high-throughput culturing approach to investigate microbial colonization of ICU sink drains. The application of culturomics entails the use of multiple culture conditions and matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF). We used WASPLab® in order to increase traceability and reproducibility and to decrease workload. The goal of this study was to relate the presence of VIM-producing P. aeruginosa to other microorganisms.
Methods
Fifteen ICU sink drains were sampled with an Eswab®. An aerobic blood culture bottle with sterile rumen fluid, sterile defibrinated sheep blood and a homemade supplement mix, was used for the pre-incubation. After pre-incubation, eight different aerobic and one anaerobic agar plates were inoculated. Inoculation on the agar plates of the pre-incubated samples in blood culture bottles were performed for one month on different incubation days: 1, 5, 10 and 21. A WASPLab®-system was used to process the samples.
Results
Approximately 3000 colonies were identified using the MALDI Biotyper®-system enabling us to identify 32 different bacterial and 4 different fungal species. In our preliminary data, seven of the 15 sink drains tested positive for VIM-producing P. aeruginosa and four of the 15 showed the presence of Candida species. Notably, Candida species and VIM-positive P. aeruginosa never occurred together. A significant (P=0.029) difference in the occurrence/association between the absence of VIM-producing P. aeruginosa in presence of Candida species was observed.
Conclusions
Our preliminary data suggests that VIM-producing P. aeruginosa occurs less frequent in sink drains colonized with Candida species.
Hospital-acquired infections, caused by multi-drug resistant, Verona Integron-encoded Metallo-β-lactamase (VIM)-producing P. aeruginosa, are a major problem in intensive care units (ICU). Often, these VIM-producing P. aeruginosa strains are present in the sink drains of the ICU and are nearly impossible to eradicate. Recent studies suggest that certain other microorganisms present in these sink drains could inhibit the growth of VIM-producing P. aeruginosa. In our study set-up, we apply a high-throughput culturing approach to investigate microbial colonization of ICU sink drains. The application of culturomics entails the use of multiple culture conditions and matrix-assisted laser desorption/ionization–time of flight (MALDI-TOF). We used WASPLab® in order to increase traceability and reproducibility and to decrease workload. The goal of this study was to relate the presence of VIM-producing P. aeruginosa to other microorganisms.
Methods
Fifteen ICU sink drains were sampled with an Eswab®. An aerobic blood culture bottle with sterile rumen fluid, sterile defibrinated sheep blood and a homemade supplement mix, was used for the pre-incubation. After pre-incubation, eight different aerobic and one anaerobic agar plates were inoculated. Inoculation on the agar plates of the pre-incubated samples in blood culture bottles were performed for one month on different incubation days: 1, 5, 10 and 21. A WASPLab®-system was used to process the samples.
Results
Approximately 3000 colonies were identified using the MALDI Biotyper®-system enabling us to identify 32 different bacterial and 4 different fungal species. In our preliminary data, seven of the 15 sink drains tested positive for VIM-producing P. aeruginosa and four of the 15 showed the presence of Candida species. Notably, Candida species and VIM-positive P. aeruginosa never occurred together. A significant (P=0.029) difference in the occurrence/association between the absence of VIM-producing P. aeruginosa in presence of Candida species was observed.
Conclusions
Our preliminary data suggests that VIM-producing P. aeruginosa occurs less frequent in sink drains colonized with Candida species.
| Original language | English |
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| Publication status | Published - 2022 |
| Event | 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) - Lisbon, Portugal Duration: 23 Apr 2022 → 26 Apr 2022 https://www.eccmid.org/ |
Conference
| Conference | 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) |
|---|---|
| Country/Territory | Portugal |
| City | Lisbon |
| Period | 23/04/22 → 26/04/22 |
| Internet address |