Preparation of Scaffolds from Decellularized Testicular Matrix

Yoni Baert; Ellen Goossens

doi:10.1007/7651_2017_29

Preparation of Scaffolds from Decellularized Testicular Matrix

Yoni Baert, Ellen Goossens

Basic (bio-) Medical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

16 Citations (Scopus)

114 Downloads (Pure)

Abstract

Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	121-127
Number of pages	7
Volume	1577
ISBN (Electronic)	1940-6029
DOIs	https://doi.org/10.1007/7651_2017_29
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology: Experimental Cholestasis Research
Publisher	Humana Press
ISSN (Print)	1064-3745

Keywords

Equipment Design
Extracellular Matrix/chemistry
Extracellular Matrix Proteins/analysis
Humans
Male
Microtomy/instrumentation
Spermatogenesis
Testis/chemistry
Tissue Engineering/instrumentation
Tissue Scaffolds/chemistry

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10.1007/7651_2017_29

53971702Accepted author manuscript, 673 KBLicence: Other

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title = "Preparation of Scaffolds from Decellularized Testicular Matrix",

abstract = "Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.",

keywords = "Equipment Design, Extracellular Matrix/chemistry, Extracellular Matrix Proteins/analysis, Humans, Male, Microtomy/instrumentation, Spermatogenesis, Testis/chemistry, Tissue Engineering/instrumentation, Tissue Scaffolds/chemistry",

author = "Yoni Baert and Ellen Goossens",

year = "2018",

doi = "10.1007/7651_2017_29",

language = "English",

isbn = "1064-3745",

volume = "1577",

series = " Methods in Molecular Biology: Experimental Cholestasis Research ",

publisher = "Humana Press",

pages = "121--127",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Preparation of Scaffolds from Decellularized Testicular Matrix

AU - Baert, Yoni

AU - Goossens, Ellen

PY - 2018

Y1 - 2018

N2 - Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.

AB - Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the preparation of decellularized testicular matrix (DTM) scaffolds with minimal damage to the native three-dimensional structure and tissue-specific ECM components. Such DTM scaffolds can help to gain a better insight into the molecular composition and function of testicular ECM and to develop new tissue engineering approaches to treat various types of male fertility disorders.

KW - Equipment Design

KW - Extracellular Matrix/chemistry

KW - Extracellular Matrix Proteins/analysis

KW - Humans

KW - Male

KW - Microtomy/instrumentation

KW - Spermatogenesis

KW - Testis/chemistry

KW - Tissue Engineering/instrumentation

KW - Tissue Scaffolds/chemistry

UR - https://link.springer.com/protocol/10.1007/7651_2017_29

U2 - 10.1007/7651_2017_29

DO - 10.1007/7651_2017_29

M3 - Chapter

C2 - 28456952

SN - 1064-3745

VL - 1577

T3 - Methods in Molecular Biology: Experimental Cholestasis Research

SP - 121

EP - 127

BT - Methods in Molecular Biology

ER -

Preparation of Scaffolds from Decellularized Testicular Matrix

Abstract

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OZRMETH3: Research at the interface between human genetics and reproduction.

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Preparation of Scaffolds from Decellularized Testicular Matrix

Abstract

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Keywords

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OZRMETH3: Research at the interface between human genetics and reproduction.

Cite this