Preparation of transgenic plants

Dimitri Verweire (Inventor)

Research output: Patent

Abstract

Selectable marker genes are used in nearly all transformation procedures. They are required for efficient generation of transgenic plants, but serve no purpose once plants have been obtained that are homozygous for the transgene. On the contrary, their continued presence can pose technological problems, because it precludes retransformation (gene-stacking) with the same marker system, and can raise safety and public concerns.

Transgenic plants become destined to lose the selectable marker when its presence is not longer required without the need of extra handling and in the same time frame as compared to transformation protocols in which the marker is not removed by introducing a genetic program (Germline-specific auto-excision Plant Transformation vector):

o Recombinase gene under control of a single germline-specific promoter
o Double selection system
o Auto-excision construct (cre gene between target sites for recombinase)
Original languageEnglish
Patent numberPCT/EP2007/060902
Publication statusPublished - 1970

Keywords

  • marker-free transgenic plants
  • site-specific recombinase

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