Purification and crystallization of Phd, the antitoxin of the phd/doc operon

Abel Garcia Pino; Yann Sterckx; Guy Vandenbussche; Remy Loris

Purification and crystallization of Phd, the antitoxin of the phd/doc operon

Abel Garcia Pino, Yann Sterckx, Guy Vandenbussche, Remy Loris

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	Acta Crystallogr F Struct Biol Commun
Volume	66
Publication status	Published - 2010

Keywords

Plasmid addiction
Stress regulation in bacteria
structural biology
Toxin-antitoxin modules
transcription regulator

FWOAL381: Structural basis of plasmide addiction and programed cell death in bacteria
Haesaerts, S., De Greve, H. & Loris, R.
1/01/06 → 31/12/09
Project: Fundamental

3 Talk or presentation at a conference
3 Talk or presentation at a workshop/seminar
2 Participation in conference

ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions
Abel Garcia Pino (Participant)
20 May 2013 → 25 May 2013
Activity: Participating in or organising an event › Participation in conference
ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions
Steven De Gieter (Speaker)
20 May 2013 → 25 May 2013
Activity: Talk or presentation › Talk or presentation at a conference
VIB Group Leader meeting
Remy Loris (Keynote speaker)
27 Jan 2012
Activity: Talk or presentation › Talk or presentation at a workshop/seminar

Cite this

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title = "Purification and crystallization of Phd, the antitoxin of the phd/doc operon",

abstract = "The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.",

keywords = "Plasmid addiction, Stress regulation in bacteria, structural biology, Toxin-antitoxin modules, transcription regulator",

author = "{Garcia Pino}, Abel and Yann Sterckx and Guy Vandenbussche and Remy Loris",

year = "2010",

language = "English",

volume = "66",

pages = "167--171",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Purification and crystallization of Phd, the antitoxin of the phd/doc operon

AU - Garcia Pino, Abel

AU - Sterckx, Yann

AU - Vandenbussche, Guy

AU - Loris, Remy

PY - 2010

Y1 - 2010

N2 - The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

AB - The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

KW - Plasmid addiction

KW - Stress regulation in bacteria

KW - structural biology

KW - Toxin-antitoxin modules

KW - transcription regulator

M3 - Article

VL - 66

SP - 167

EP - 171

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

SN - 1744-3091

ER -

Purification and crystallization of Phd, the antitoxin of the phd/doc operon

Abstract

Keywords

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Projects

FWOAL381: Structural basis of plasmide addiction and programed cell death in bacteria

Activities

ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions

ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions

VIB Group Leader meeting

Cite this