Abstract
The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.
| Original language | English |
|---|---|
| Pages (from-to) | 167-171 |
| Number of pages | 5 |
| Journal | Acta Crystallogr F Struct Biol Commun |
| Volume | 66 |
| Publication status | Published - 2010 |
Keywords
- Plasmid addiction
- Stress regulation in bacteria
- structural biology
- Toxin-antitoxin modules
- transcription regulator
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Dive into the research topics of 'Purification and crystallization of Phd, the antitoxin of the phd/doc operon'. Together they form a unique fingerprint.Projects
- 1 Finished
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FWOAL381: Structural basis of plasmide addiction and programed cell death in bacteria
Haesaerts, S. (Mandate), De Greve, H. (Co-Promotor) & Loris, R. (Administrative Promotor)
1/01/06 → 31/12/09
Project: Fundamental
Activities
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ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions
De Gieter, S. (Speaker)
20 May 2013 → 25 May 2013Activity: Talk or presentation › Talk or presentation at a conference
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ESF-EMBO Symposium on Molecular Perspectives On Protein-Protein Interactions
Garcia Pino, A. (Participant)
20 May 2013 → 25 May 2013Activity: Participating in or organising an event › Participation in conference
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VIB Group Leader meeting
Loris, R. (Keynote speaker)
27 Jan 2012Activity: Talk or presentation › Talk or presentation at a workshop/seminar
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