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Abstract
Mimicking the in vivo microenvironment is one of the current strategies to maintain liver-specific functionality in primary cultured hepatocytes for long periods. Freshly isolated hepatocytes entrapped in collagen gel type I (collagen gel immobilization culture) or sandwiched between two layers of hydrated collagen type I (collagen gel sandwich culture) are known to display liver-specific functions (e.g., biotransformation capacity) for more than 6 wk. We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture.
| Original language | English |
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| Title of host publication | Cytochrome P450 Protocols |
| Publisher | In: Cytochrome P450 Protocols. Series in: Methods in Molecualr Biology. Vol. 320, Chap. 28, pp. 247-254, 2006 |
| Pages | 247-254 |
| Number of pages | 8 |
| Volume | 320 |
| ISBN (Print) | 978-1-58829-441-8 |
| Publication status | Published - 1 May 2006 |
Publication series
| Name | Methods in Molecular Biology |
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Bibliographical note
In: Cytochrome P450 Protocols. Series in: Methods in Molecualr Biology. Vol. 320, Chap. 28, pp. 247-254, 2006Fingerprint
Dive into the research topics of 'Rat hepatocytes cultures: collagen gel sandwich and immobilization cultures'. Together they form a unique fingerprint.Activities
- 1 Talk or presentation at a conference
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CBTOX XVIII Brazilian Congress of Toxicology
Mathieu Vinken (Keynote speaker)
7 Oct 2013 → 10 Oct 2013Activity: Talk or presentation › Talk or presentation at a conference