Abstract
We studied two exposed groups of nuclear plant workers, one
chronically exposed (n=28) and the other made up of seasonal
cleaners acutely exposed (n=32). The third and fourth groups
were controls to the first (n=19) and second (n=31) groups
respectively made of office staf f. Using the Comet assay, we
assessed DNA damage and the DNA strand break repair
phenotype. The frequencies of micronuclei were assessed by
means of the in vitro micronucleus assay. Genotyping for
DNA repair genes OGG1, XRCC1 and XRCC3 was
performed on blood samples of the acutely exposed workers
using restricted fragment length polymorphis (RFLP).
Our data show that exposed workers repaired damage to their
DNA more proficiently than their controls. Also, the exposed
smokers had higher levels of DNA damage and micronuclei
frequencies than non smokers. In the acutely exposed
workers, a significant contribution of the OGG1 genotypes
to the in vitro DNA strand break repair capacity was found. A
multivariate analysis revealed that genetic polymorphisms in
XRCC1 resulted in higher residual DNA values and the
Met/Met variant of XRCC3 gave an increased frequency of
micronuclei. We conclude that a combined analysis of the
three genotypes, OGG1, XRCC1 and XRCC3 polymorphisms
is advised in order to assess individual susceptibility to
ionising radiation. As an alternative or complement, the in
vitro DNA strand break repair phenotype which integrates
several repair pathways is recommended.
chronically exposed (n=28) and the other made up of seasonal
cleaners acutely exposed (n=32). The third and fourth groups
were controls to the first (n=19) and second (n=31) groups
respectively made of office staf f. Using the Comet assay, we
assessed DNA damage and the DNA strand break repair
phenotype. The frequencies of micronuclei were assessed by
means of the in vitro micronucleus assay. Genotyping for
DNA repair genes OGG1, XRCC1 and XRCC3 was
performed on blood samples of the acutely exposed workers
using restricted fragment length polymorphis (RFLP).
Our data show that exposed workers repaired damage to their
DNA more proficiently than their controls. Also, the exposed
smokers had higher levels of DNA damage and micronuclei
frequencies than non smokers. In the acutely exposed
workers, a significant contribution of the OGG1 genotypes
to the in vitro DNA strand break repair capacity was found. A
multivariate analysis revealed that genetic polymorphisms in
XRCC1 resulted in higher residual DNA values and the
Met/Met variant of XRCC3 gave an increased frequency of
micronuclei. We conclude that a combined analysis of the
three genotypes, OGG1, XRCC1 and XRCC3 polymorphisms
is advised in order to assess individual susceptibility to
ionising radiation. As an alternative or complement, the in
vitro DNA strand break repair phenotype which integrates
several repair pathways is recommended.
| Original language | English |
|---|---|
| Title of host publication | GENETIC EPIDEMIOLOGY |
| Pages | 614-615 |
| Number of pages <span style="color:red"p> <font size="1.5"> ✽ </span> </font> | 2 |
| Volume | 31 |
| Publication status | Published - Sep 2007 |
| Event | Finds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet - Stockholm, Sweden Duration: 21 Sep 2009 → 25 Sep 2009 |
Publication series
| Name | GENETIC EPIDEMIOLOGY |
|---|---|
| Number | 6 |
Conference
| Conference | Finds and Results from the Swedish Cyprus Expedition: A Gender Perspective at the Medelhavsmuseet |
|---|---|
| Country/Territory | Sweden |
| City | Stockholm |
| Period | 21/09/09 → 25/09/09 |
Keywords
- repair phenotype
- polymorphisms
- micronuclei
- DNA damage