Resolving power in liquid chromatography: A trade-off between efficiency and analysis time

Jose Luis Dores-Sousa; Jelle De Vos; Sebastiaan Eeltink

doi:10.1002/jssc.201800891

Resolving power in liquid chromatography: A trade-off between efficiency and analysis time

Jose Luis Dores-Sousa, Jelle De Vos, Sebastiaan Eeltink

Research output: Contribution to journal › Scientific review › peer-review

19 Citations (Scopus)

190 Downloads (Pure)

Abstract

This review describes chromatographic dispersion and different plate-height models frequently used to assess the chromatographic performance of ultra-high-pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column-technology developments to advance the kinetic performance limits are discussed and selected state-of-the-art applications are highlighted.

Original language	English
Pages (from-to)	38-50
Number of pages	13
Journal	Journal of Separation Science
Volume	42
Issue number	1
DOIs	https://doi.org/10.1002/jssc.201800891
Publication status	Published - Jan 2019

Keywords

kinetic performance
kinetic plot
ultra high performance liquid chromatography
ultra high pressure liquid chromatography
van Deemter curve

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10.1002/jssc.201800891

Manuscript_Dores-Sousa et al JSS 2019Accepted author manuscript, 710 KB

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abstract = "This review describes chromatographic dispersion and different plate-height models frequently used to assess the chromatographic performance of ultra-high-pressure liquid chromatography column technology. Furthermore, different performance indices, including the resolution, the separation impedance, and kinetic plots are discussed allowing to quantify and visualize the resolving power in liquid chromatography. The construction of kinetic plots is explained, and different visualization approaches are highlighted. Finally, key instrument and column-technology developments to advance the kinetic performance limits are discussed and selected state-of-the-art applications are highlighted.",

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Resolving power in liquid chromatography: A trade-off between efficiency and analysis time

Abstract

Keywords

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SRP6: Strategic Research Programme: Exploiting the Advantages of Order and Geometrical Structure for a Greener Chemistry

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Resolving power in liquid chromatography: A trade-off between efficiency and analysis time

Abstract

Keywords

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Other files and links

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Projects

SRP6: Strategic Research Programme: Exploiting the Advantages of Order and Geometrical Structure for a Greener Chemistry

Cite this