Reversing complete mechanical transzonal projections disruption during mouse in vitro follicle culture with unaltered oocyte competence

Research output: Contribution to journalArticle

Abstract

In vitro oocyte growth is widely studied as an alternative fertility preservation approach. Several animal models are used to generate extensive information on this complex process regulated by the constant and dynamic interaction between the oocyte and its somatic compartment throughout follicle growth and maturation. A 2-dimensional (2D) attachment mouse secondary follicle culture system was used to assess the oocyte's capacity to overcome disconnection from its somatic companions at different developmental stages for final competence acquisition. To test this, complete mechanical denudation of oocytes from preantral and early antral follicles was performed. Established endpoints were the oocyte's potential to reconnect with somatic cells and the impact of connectivity disruption on mature oocyte quality. This study proves that oocytes from preantral and early antral cultured mouse follicles can overcome complete denudation, restoring likely functional transzonal projections (TZPs) with no significant differences in meiotic and developmental competence compared to those from intact cultured follicles. These novel findings constitute good premises for developing successful strategies to rescue human oocyte competence in the context of in vitro culture approaches such as non-hCG triggered in vitro maturation (IVM).

Original languageEnglish
Pages (from-to)1373-1385
Number of pages13
JournalBiology of Reproduction
Volume104
Issue number6
Early online date2021
DOIs
Publication statusPublished - 4 Jun 2021

Bibliographical note

© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Keywords

  • antral follicle
  • in vitro follicle culture
  • oocyte competence
  • oocyte-somatic reconnection
  • transzonal projections

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