Robotic Cell Printing for Constructing Living Yeast Cell Microarrays in Microfluidic Chips

Research output: Contribution to journalArticle

3 Citations (Scopus)
78 Downloads (Pure)

Abstract

Living cell microarrays in microfluidic chips allow the non-invasive multiplexed molecular analysis of single cells. Here, we developed a simple and affordable perfusion microfluidic chip containing a living yeast cell array composed of a population of cell variants (green fluorescent protein (GFP)-tagged Saccharomyces cerevisiae clones). We combined mechanical patterning in 102 microwells and robotic piezoelectric cell dispensing in the microwells to construct the cell arrays. Robotic yeast cell dispensing of a yeast collection from a multiwell plate to the microfluidic chip microwells was optimized. The developed microfluidic chip and procedure were validated by observing the growth of GFP-tagged yeast clones that are linked to the cell cycle by time-lapse fluorescence microscopy over a few generations. The developed microfluidic technology has the potential to be easily upscaled to a high-density cell array allowing us to perform dynamic proteomics and localizomics experiments.

Original languageEnglish
Article number26
Pages (from-to)1-14
Number of pages14
JournalFermentation
Volume6
Issue number1
DOIs
Publication statusPublished - 14 Feb 2020

Fingerprint

Dive into the research topics of 'Robotic Cell Printing for Constructing Living Yeast Cell Microarrays in Microfluidic Chips'. Together they form a unique fingerprint.

Cite this