Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.

Annelies De Bel; Deborah De Geyter; Iris De Schutter; I Wellemans; Charlotte Mouton; L Hanssens; Petra Schelstraete; Anne Malfroot; Denis Pierard

Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.

Annelies De Bel, Deborah De Geyter, Iris De Schutter, I Wellemans, Charlotte Mouton, L Hanssens, Petra Schelstraete, Anne Malfroot, Denis Pierard

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-L-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.

Original language	English
Pages (from-to)	4204-4206
Number of pages <span style="color:red"p> <font size="1.5"> ✽ </span> </font>	3
Journal	J Clin Microbiol
Volume	51
Issue number	December
Publication status	Published - 2013

Keywords

cystic fibrosis
Transport-systems
recovery
swabs

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De Bel, A., De Geyter, D., De Schutter, I., Wellemans, I., Mouton, C., Hanssens, L., Schelstraete, P., Malfroot, A., & Pierard, D. (2013). Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients. J Clin Microbiol, 51(December), 4204-4206. http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=4&SID=Z1CpKFNgAm6hgAXzTJW&page=1&doc=5

@article{a0f89e1361594d11b7e7ad8e63b525ae,

title = "Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.",

abstract = "We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-L-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.",

keywords = "cystic fibrosis, Transport-systems, recovery, swabs",

author = "{De Bel}, Annelies and {De Geyter}, Deborah and {De Schutter}, Iris and I Wellemans and Charlotte Mouton and L Hanssens and Petra Schelstraete and Anne Malfroot and Denis Pierard",

year = "2013",

language = "English",

volume = "51",

pages = "4204--4206",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "December",

}

De Bel, A, De Geyter, D, De Schutter, I, Wellemans, I, Mouton, C, Hanssens, L, Schelstraete, P, Malfroot, A & Pierard, D 2013, 'Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.', J Clin Microbiol, vol. 51, no. December, pp. 4204-4206. <http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=4&SID=Z1CpKFNgAm6hgAXzTJW&page=1&doc=5>

Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients. / De Bel, Annelies; De Geyter, Deborah; De Schutter, Iris; Wellemans, I; Mouton, Charlotte; Hanssens, L; Schelstraete, Petra; Malfroot, Anne ; Pierard, Denis.

In: J Clin Microbiol, Vol. 51, No. December, 2013, p. 4204-4206.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.

AU - De Bel, Annelies

AU - De Geyter, Deborah

AU - De Schutter, Iris

AU - Wellemans, I

AU - Mouton, Charlotte

AU - Hanssens, L

AU - Schelstraete, Petra

AU - Malfroot, Anne

AU - Pierard, Denis

PY - 2013

Y1 - 2013

N2 - We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-L-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.

AB - We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-L-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.

KW - cystic fibrosis

KW - Transport-systems

KW - recovery

KW - swabs

M3 - Article

VL - 51

SP - 4204

EP - 4206

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - December

ER -

Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients.

Abstract

Keywords

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