Screening of endocrine activity of compounds migrating from plastic baby bottles using a multi-receptor panel of in vitro bioassays

Coraline Simon, Matthias Onghena, Adrian Covaci, Els Van Hoeck, Joris Van Loco, Tara Vandermarken, Kersten Van Langenhove, Heidi Demaegdt, Birgit Mertens, Karine Vandermeiren, Mi Scippo, Marc Elskens

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Endocrine activity of 65compounds migrating from polycarbonate replacement plasticbabybottles was assessed using in vitro cell based assays (reporter gene assays) involving 7 nuclear receptors, i.e. human steroid hormones receptors (oestrogen, androgen, progesterone and glucocorticoid receptors), human thyroid beta and peroxi-some proliferator-activated gamma receptors, and the mouse aryl hydrocarbon receptor. The chemicals were tested at 4 concentrations ranging from 0.001 mM to 1 mM. Only twelve chemicals did not show any activity towards any of the nuclear receptors, while fifty three compounds showed a possible endocrine activity. Most of the agonistic activities were observed towards the oestrogen receptor while the PPARγ
was the target for most of the recorded antagonistic activities. Agonistic activities were recorded for several phthalates, benzophenones, aromatic hydrocarbons and phenols, while compounds such as benzaldehydes, ketones and esters of fatty acid showed antagonistic activities. Thirty five chemicals were able of agonistic activities on 1 to 4 receptors and antagonistic activities were recorded for 35 compounds as well, towards 1 to 7 receptors. Sixteen compounds were able of both agonistic and antagonistic activities, but not on the same receptors, except in 2 cases for the oestrogen receptor and 4 cases for the PPARγ.
Original languageEnglish
Pages (from-to)121-133
Number of pages13
JournalToxicology in Vitro
Volume37
Early online date12 Sep 2016
DOIs
Publication statusPublished - 17 Oct 2016

Keywords

  • Food contact material
  • Endocrine disruptor
  • Endocrine active substances
  • Reporter gene assay
  • Nuclear receptors

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