Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide

Catarina Xavier; Nick Devoogdt; Sophie Hernot; Ilse Vaneycken; Matthias D'Huyvetter; Jens De Vos; Sam Massa; Tony Lahoutte; Vicky Caveliers

doi:10.1007/978-1-61779-968-6_30

Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide

Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, Vicky Caveliers

Medical Imaging and Physical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

42 Citations (Scopus)

Abstract

99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.

Original language	English
Title of host publication	Single Domain Antibodies
Subtitle of host publication	Methods and Protocols
Editors	Dirk Saerens, Serge Muyldermans
Publisher	Humana Press
Pages	485-490
Number of pages	6
ISBN (Electronic)	978-1-61779-968-6
ISBN (Print)	978-1-61779-967-9
DOIs	https://doi.org/10.1007/978-1-61779-968-6_30
Publication status	Published - 10 Aug 2012

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	911
ISSN (Print)	1064-3745

Bibliographical note

Dirk Saerens and Serge Muyldermans

Keywords

Nanobodies

Access to Document

10.1007/978-1-61779-968-6_30

Cite this

Xavier, C., Devoogdt, N., Hernot, S., Vaneycken, I., D'Huyvetter, M., De Vos, J., Massa, S., Lahoutte, T., & Caveliers, V. (2012). Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide. In D. Saerens, & S. Muyldermans (Eds.), Single Domain Antibodies: Methods and Protocols (pp. 485-490). (Methods in Molecular Biology; Vol. 911). Humana Press. https://doi.org/10.1007/978-1-61779-968-6_30

Xavier, Catarina ; Devoogdt, Nick ; Hernot, Sophie ; Vaneycken, Ilse ; D'Huyvetter, Matthias ; De Vos, Jens ; Massa, Sam ; Lahoutte, Tony ; Caveliers, Vicky. / Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide. Single Domain Antibodies: Methods and Protocols. editor / Dirk Saerens ; Serge Muyldermans. Humana Press, 2012. pp. 485-490 (Methods in Molecular Biology).

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abstract = "99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.",

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Xavier, C, Devoogdt, N , Hernot, S , Vaneycken, I , D'Huyvetter, M, De Vos, J, Massa, S, Lahoutte, T & Caveliers, V 2012, Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide. in D Saerens & S Muyldermans (eds), Single Domain Antibodies: Methods and Protocols. Methods in Molecular Biology, vol. 911, Humana Press, pp. 485-490. https://doi.org/10.1007/978-1-61779-968-6_30

Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide. / Xavier, Catarina; Devoogdt, Nick ; Hernot, Sophie ; Vaneycken, Ilse ; D'Huyvetter, Matthias; De Vos, Jens; Massa, Sam; Lahoutte, Tony ; Caveliers, Vicky.

Single Domain Antibodies: Methods and Protocols. ed. / Dirk Saerens; Serge Muyldermans. Humana Press, 2012. p. 485-490 (Methods in Molecular Biology; Vol. 911).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Site-specific labeling of his-tagged Nanobodies with 99mTc: A practical guide

Abstract

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Keywords

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