Slc7a11 (xCT) protein expression is not altered in the depressed brain and system xc- deficiency does not affect depression-associated behaviour in the corticosterone mouse model

Thomas Demuyser, Lauren Deneyer, Eduard Bentea, Giulia Albertini, Teresa Femenia, Laura Walrave, Hideyo Sato, Niels C Danbolt, Dimitri De Bundel, Alex Michotte, Maria Lindskog, Ann Massie, Ilse Smolders

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

OBJECTIVES: The cystine/glutamate antiporter (system xc-) is believed to contribute to nonvesicular glutamate release from glial cells in various brain areas. Although recent investigations implicate system xc- in mood disorders, unambiguous evidence has not yet been established. Therefore, we evaluated the possible role of system xc- in the depressive state.

METHODS: We conducted a protein expression analysis of the specific subunit of system xc- (xCT) in brain regions of the corticosterone mouse model, Flinders Sensitive Line rat model and post-mortem tissue of depressed patients. We next subjected system xc- deficient mice to the corticosterone model and analysed their behaviour in several tests. Lastly, we subjected additional cohorts of xCT-deficient and wild-type mice to N-acetylcysteine treatment to unveil whether the previously reported antidepressant-like effects are dependent upon system xc-.

RESULTS: We did not detect any changes in xCT expression levels in the animal models or patients compared to proper controls. Furthermore, loss of system xc- had no effect on depression- and anxiety-like behaviour. Finally, the antidepressant-like effects of N-acetylcysteine are not mediated via system xc-.

CONCLUSIONS: xCT protein expression is not altered in the depressed brain and system xc- deficiency does not affect depression-associated behaviour in the corticosterone mouse model.

Original languageEnglish
Pages (from-to)381-392
Number of pages12
JournalWorld Journal of Biological Psychiatry
Volume20
Issue number5
Early online date27 Sep 2017
DOIs
Publication statusPublished - Jun 2019

Keywords

  • Journal Article
  • SIc7a11
  • protein expression
  • depressed brain
  • depression-associated behaviour
  • corticosterone mouse model

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