Spiral-Mediated Growth Can Lead to Crystals of Higher Purity

Mike Sleutel; Gen Sazaki; Alexander Van Driessche

Spiral-Mediated Growth Can Lead to Crystals of Higher Purity

Mike Sleutel, Gen Sazaki, Alexander Van Driessche

Department of Bio-engineering Sciences

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The morphology and step kinetics of 2D islands and
spiral hillocks of lysozyme crystals growing from purified and
contaminated solutions were determined and compared. It was found
that the morphology and step dynamics of spiral hillocks of lysozyme
crystals are less affected by the presence of impurities in the growth
solution as compared to steps generated by 2D nucleation. These
observations could be satisfactorily explained considering the terrace
exposure time of spiral hillocks (NLGÑsp) and 2D islands (NLGÑ2D) and the
characteristic impurity adsorption time (NLGÑi). For lysozyme, overlapping
time scales of terrace exposure and impurity adsorption exist
and NLGÑi . NLGÑsp <NLGÑ2D. Hence, when crystal growth is dominated by spiral
hillocks, less impurities are adsorbed onto the crystal surface and a
more pure crystal lattice is formed. Although spiral hillocks reduce
the effect of impurities, they do play a significant role in the mechanism of step bunching.

Original language	English
Pages (from-to)	2367-2374
Journal	Crystal Growth & Design
Volume	12
Issue number	5
Publication status	Published - 2012

Keywords

protein
crystal growth
impurity effects
spiral dislocation
two-dimensional nucleation

Cite this

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title = "Spiral-Mediated Growth Can Lead to Crystals of Higher Purity",

abstract = "The morphology and step kinetics of 2D islands and spiral hillocks of lysozyme crystals growing from purified and contaminated solutions were determined and compared. It was found that the morphology and step dynamics of spiral hillocks of lysozyme crystals are less affected by the presence of impurities in the growth solution as compared to steps generated by 2D nucleation. These observations could be satisfactorily explained considering the terrace exposure time of spiral hillocks (NLG{\~N}sp) and 2D islands (NLG{\~N}2D) and the characteristic impurity adsorption time (NLG{\~N}i). For lysozyme, overlapping time scales of terrace exposure and impurity adsorption exist and NLG{\~N}i . NLG{\~N}sp <NLG{\~N}2D. Hence, when crystal growth is dominated by spiral hillocks, less impurities are adsorbed onto the crystal surface and a more pure crystal lattice is formed. Although spiral hillocks reduce the effect of impurities, they do play a significant role in the mechanism of step bunching.",

keywords = "protein, crystal growth, impurity effects, spiral dislocation, two-dimensional nucleation",

author = "Mike Sleutel and Gen Sazaki and {Van Driessche}, Alexander",

year = "2012",

language = "English",

volume = "12",

pages = "2367--2374",

journal = "Crystal Growth & Design",

issn = "1528-7483",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Spiral-Mediated Growth Can Lead to Crystals of Higher Purity

AU - Sleutel, Mike

AU - Sazaki, Gen

AU - Van Driessche, Alexander

PY - 2012

Y1 - 2012

N2 - The morphology and step kinetics of 2D islands and spiral hillocks of lysozyme crystals growing from purified and contaminated solutions were determined and compared. It was found that the morphology and step dynamics of spiral hillocks of lysozyme crystals are less affected by the presence of impurities in the growth solution as compared to steps generated by 2D nucleation. These observations could be satisfactorily explained considering the terrace exposure time of spiral hillocks (NLGÑsp) and 2D islands (NLGÑ2D) and the characteristic impurity adsorption time (NLGÑi). For lysozyme, overlapping time scales of terrace exposure and impurity adsorption exist and NLGÑi . NLGÑsp <NLGÑ2D. Hence, when crystal growth is dominated by spiral hillocks, less impurities are adsorbed onto the crystal surface and a more pure crystal lattice is formed. Although spiral hillocks reduce the effect of impurities, they do play a significant role in the mechanism of step bunching.

AB - The morphology and step kinetics of 2D islands and spiral hillocks of lysozyme crystals growing from purified and contaminated solutions were determined and compared. It was found that the morphology and step dynamics of spiral hillocks of lysozyme crystals are less affected by the presence of impurities in the growth solution as compared to steps generated by 2D nucleation. These observations could be satisfactorily explained considering the terrace exposure time of spiral hillocks (NLGÑsp) and 2D islands (NLGÑ2D) and the characteristic impurity adsorption time (NLGÑi). For lysozyme, overlapping time scales of terrace exposure and impurity adsorption exist and NLGÑi . NLGÑsp <NLGÑ2D. Hence, when crystal growth is dominated by spiral hillocks, less impurities are adsorbed onto the crystal surface and a more pure crystal lattice is formed. Although spiral hillocks reduce the effect of impurities, they do play a significant role in the mechanism of step bunching.

KW - protein

KW - crystal growth

KW - impurity effects

KW - spiral dislocation

KW - two-dimensional nucleation

M3 - Article

SN - 1528-7483

VL - 12

SP - 2367

EP - 2374

JO - Crystal Growth & Design

JF - Crystal Growth & Design

IS - 5

ER -

Spiral-Mediated Growth Can Lead to Crystals of Higher Purity

Abstract

Keywords

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