Structural Characterization of Monomeric/Dimeric State of p59(fyn) SH2 Domain

Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, Tom Lenaerts

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59(fyn) SH2 domain.

Original languageEnglish
Pages (from-to)257-267
Number of pages11
JournalMethods in Molecular Biology
Volume1555
DOIs
Publication statusPublished - 15 Jan 2017

Keywords

  • Circular dichroism (CD)
  • Gel filtration chromatography
  • Monomer/dimer
  • Nuclear magnetic resonance (NMR)
  • SH2 domain

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