Structural insights into the intertwined dimer of Fyn SH2

Radu Ion Huculeci, Abel Garcia Pino, Lieven Buts, Tom Lenaerts, Nico Van Nuland

Research output: Contribution to journalArticle

5 Citations (Scopus)


Src homology 2 domains are interaction modules dedicated to the recognition of phosphotyrosine sites incorporated in numerous proteins found in intracellular signaling pathways. Here we provide for the first time structural insight into the dimerization of Fyn SH2 both in solution and in crystalline conditions, providing novel crystal structures of both the dimer and peptide-bound structures of Fyn SH2. Using nuclear magnetic resonance chemical shift analysis, we show how the peptide is able to eradicate the dimerization, leading to monomeric SH2 in its bound state. Furthermore, we show that Fyn SH2's dimer form differs from other SH2 dimers reported earlier. Interestingly, the Fyn dimer can be used to construct a completed dimer model of Fyn without any steric clashes. Together these results extend our understanding of SH2 dimerization, giving structural details, on one hand, and suggesting a possible physiological relevance of such behavior, on the other hand.
Original languageEnglish
Pages (from-to)1964-1978
Number of pages15
JournalProtein Science
Issue number12
Early online date7 Oct 2015
Publication statusPublished - Dec 2015

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