Structure of a bacterial type IV secretion core complex at subnanometre resolution

Angel Rivera-Calzada; Rémi Fronzes; Christos G Savva; Vidya Chandran; Pei W Lian; Toon Laeremans; Els Pardon; Jan Steyaert; Han Karel Remaut; G. Waksman; Elena V Orlova

doi:10.1038/emboj. 2013.58

Structure of a bacterial type IV secretion core complex at subnanometre resolution

Angel Rivera-Calzada, Rémi Fronzes, Christos G Savva, Vidya Chandran, Pei W Lian, Toon Laeremans, Els Pardon, Jan Steyaert, Han Karel Remaut, G. Waksman, Elena V Orlova

Department of Bio-engineering Sciences

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

72 Downloads (Pure)

Abstract

Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

Original language	English
Pages (from-to)	1195–1204
Number of pages	10
Journal	EMBO Journal
Volume	32
DOIs	https://doi.org/10.1038/emboj. 2013.58
Publication status	Published - 19 Mar 2013

Keywords

core complex
cryo electron microscopy

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10.1038/emboj. 2013.58

emboj201358aFinal published version, 2.24 MB

http://onlinelibrary.wiley.com/doi/10.1038/emboj.2013.58/full

SRP13: Strategic Research Programme: Structure and dynamics of macromolecular complexes in health and disease
Steyaert, J., Remaut, H. K., Loris, R., Efremov, R., Steyaert, J., Loris, R. & Remaut, H. K.
1/11/12 → 31/10/24
Project: Fundamental

Cite this

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title = "Structure of a bacterial type IV secretion core complex at subnanometre resolution",

abstract = "Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.",

keywords = "core complex, cryo electron microscopy",

author = "Angel Rivera-Calzada and R{\'e}mi Fronzes and Savva, {Christos G} and Vidya Chandran and Lian, {Pei W} and Toon Laeremans and Els Pardon and Jan Steyaert and Remaut, {Han Karel} and G. Waksman and Orlova, {Elena V}",

year = "2013",

month = mar,

day = "19",

doi = "10.1038/emboj. 2013.58",

language = "English",

volume = " 32",

pages = "1195–1204",

journal = "EMBO Journal",

issn = "0261-4189",

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Structure of a bacterial type IV secretion core complex at subnanometre resolution. / Rivera-Calzada, Angel; Fronzes, Rémi; Savva, Christos G; Chandran, Vidya; Lian, Pei W; Laeremans, Toon ; Pardon, Els ; Steyaert, Jan ; Remaut, Han Karel; Waksman, G.; Orlova, Elena V.

In: EMBO Journal, Vol. 32, 19.03.2013, p. 1195–1204.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Structure of a bacterial type IV secretion core complex at subnanometre resolution

AU - Rivera-Calzada, Angel

AU - Fronzes, Rémi

AU - Savva, Christos G

AU - Chandran, Vidya

AU - Lian, Pei W

AU - Laeremans, Toon

AU - Pardon, Els

AU - Steyaert, Jan

AU - Remaut, Han Karel

AU - Waksman, G.

AU - Orlova, Elena V

PY - 2013/3/19

Y1 - 2013/3/19

N2 - Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

AB - Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

KW - core complex

KW - cryo electron microscopy

U2 - 10.1038/emboj. 2013.58

DO - 10.1038/emboj. 2013.58

M3 - Article

VL - 32

SP - 1195

EP - 1204

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

ER -

Structure of a bacterial type IV secretion core complex at subnanometre resolution

Abstract

Keywords

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Projects

SRP13: Strategic Research Programme: Structure and dynamics of macromolecular complexes in health and disease

Cite this