Targeted Repolarization of Tumor-Associated Macrophages via Imidazoquinoline-Linked Nanobodies

Evangelia Bolli, Maximilian Scherger, Sana Arnouk, Ana Rita Pombo Antunes, David Straßburger, Moritz Urschbach, Judith Stickdorn, Karen De Vlaminck, Kiavash Movahedi, Hans Joachim Räder, Sophie Hernot, Pol Besenius, Jo Van Ginderachter, Lutz Nuhn

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Tumor-associated macrophages (TAMs) promote the immune suppressive microenvironment inside tumors and are, therefore, considered as a promising target for the next generation of cancer immunotherapies. To repolarize their phenotype into a tumoricidal state, the Toll-like receptor 7/8 agonist imidazoquinoline IMDQ is site-specifically and quantitatively coupled to single chain antibody fragments, so-called nanobodies, targeting the macrophage mannose receptor (MMR) on TAMs. Intravenous injection of these conjugates result in a tumor- and cell-specific delivery of IMDQ into MMRhigh TAMs, causing a significant decline in tumor growth. This is accompanied by a repolarization of TAMs towards a pro-inflammatory phenotype and an increase in anti-tumor T cell responses. Therefore, the therapeutic benefit of such nanobody-drug conjugates may pave the road towards effective macrophage re-educating cancer immunotherapies.
Original languageEnglish
Article number2004574
Number of pages12
JournalAdvanced Science
Issue number10
Publication statusPublished - 19 May 2021

Bibliographical note

Funding Information:
E.B., M.S., and S.M.A. contributed equally to this work. All authors gratefully acknowledge support by the DFG Emmy Noether program and the SFB1066. Moreover, they are grateful to the support by the FWO Flanders and Kom op tegen Kanker as well as the Fonds der Chemischen Industrie for support by their Liebig program.

Publisher Copyright:
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH

Copyright 2021 Elsevier B.V., All rights reserved.


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