Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Jaime Onofre; Prashant Kadam; Yoni Baert; Ellen Goossens

doi:10.1016/j.rbmo.2019.10.016

Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Jaime Onofre, Prashant Kadam, Yoni Baert, Ellen Goossens

Basic (bio-) Medical Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

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Abstract

RESEARCH QUESTION: Which cryopreservation method better protects reproductive potential: the cryopreservation of a testicular cell suspension (TCS) or the cryopreservation of testicular tissue (TET)?

DESIGN: Two cryopreservation strategies for spermatogonial stem cells (SSCs) were compared in a mouse model: cryopreservation as TET or as TCS. Evaluated outcomes were number of viable cells after thawing, number and length of donor-derived colonies after spermatogonial stem cell transplantation (SSCT), number of litters, litter size and number of donor-derived pups after mating.

RESULTS: Compared with cryopreserving TCS, cryopreservation of TET resulted in significantly higher numbers of viable cells after thawing (TET: 13.4  ×  104 ± 7.2  ×  104 versus TCS: 8.2  ×  104 ± 2.7  ×  104; P = 0.0002), more (TET: 47.6 ± 19.2 versus TCS: 18.5 ± 13.0; P = 0.0039) and longer (TET: 5.2 ± 1.0 mm versus TCS: 2.7 ± 1.5 mm; P = 0.0016) donor-derived colonies, and more donor-derived pups per litter (TET: 2.2 ± 0.2 versus TCS: 0.5 ± 0.1; P = 0.0008).

CONCLUSIONS: Cryopreservation of TET is the preferred method to cryopreserve SSCs prior to SSCT in a mouse model.

Original language	English
Pages (from-to)	261-269
Number of pages	9
Journal	Reproductive Biomedicine Online
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/j.rbmo.2019.10.016
Publication status	Published - Feb 2020

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10.1016/j.rbmo.2019.10.016Licence: CC BY-NC-ND

1-s2.0-S1472648319307965-mainFinal published version, 1.33 MBLicence: CC BY-NC-ND

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title = "Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation",

abstract = "RESEARCH QUESTION: Which cryopreservation method better protects reproductive potential: the cryopreservation of a testicular cell suspension (TCS) or the cryopreservation of testicular tissue (TET)?DESIGN: Two cryopreservation strategies for spermatogonial stem cells (SSCs) were compared in a mouse model: cryopreservation as TET or as TCS. Evaluated outcomes were number of viable cells after thawing, number and length of donor-derived colonies after spermatogonial stem cell transplantation (SSCT), number of litters, litter size and number of donor-derived pups after mating.RESULTS: Compared with cryopreserving TCS, cryopreservation of TET resulted in significantly higher numbers of viable cells after thawing (TET: 13.4  ×  104 ± 7.2  ×  104 versus TCS: 8.2  ×  104 ± 2.7  ×  104; P = 0.0002), more (TET: 47.6 ± 19.2 versus TCS: 18.5 ± 13.0; P = 0.0039) and longer (TET: 5.2 ± 1.0 mm versus TCS: 2.7 ± 1.5 mm; P = 0.0016) donor-derived colonies, and more donor-derived pups per litter (TET: 2.2 ± 0.2 versus TCS: 0.5 ± 0.1; P = 0.0008).CONCLUSIONS: Cryopreservation of TET is the preferred method to cryopreserve SSCs prior to SSCT in a mouse model.",

author = "Jaime Onofre and Prashant Kadam and Yoni Baert and Ellen Goossens",

year = "2020",

month = feb,

doi = "10.1016/j.rbmo.2019.10.016",

language = "English",

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AU - Onofre, Jaime

AU - Kadam, Prashant

AU - Baert, Yoni

AU - Goossens, Ellen

PY - 2020/2

Y1 - 2020/2

N2 - RESEARCH QUESTION: Which cryopreservation method better protects reproductive potential: the cryopreservation of a testicular cell suspension (TCS) or the cryopreservation of testicular tissue (TET)?DESIGN: Two cryopreservation strategies for spermatogonial stem cells (SSCs) were compared in a mouse model: cryopreservation as TET or as TCS. Evaluated outcomes were number of viable cells after thawing, number and length of donor-derived colonies after spermatogonial stem cell transplantation (SSCT), number of litters, litter size and number of donor-derived pups after mating.RESULTS: Compared with cryopreserving TCS, cryopreservation of TET resulted in significantly higher numbers of viable cells after thawing (TET: 13.4  ×  104 ± 7.2  ×  104 versus TCS: 8.2  ×  104 ± 2.7  ×  104; P = 0.0002), more (TET: 47.6 ± 19.2 versus TCS: 18.5 ± 13.0; P = 0.0039) and longer (TET: 5.2 ± 1.0 mm versus TCS: 2.7 ± 1.5 mm; P = 0.0016) donor-derived colonies, and more donor-derived pups per litter (TET: 2.2 ± 0.2 versus TCS: 0.5 ± 0.1; P = 0.0008).CONCLUSIONS: Cryopreservation of TET is the preferred method to cryopreserve SSCs prior to SSCT in a mouse model.

AB - RESEARCH QUESTION: Which cryopreservation method better protects reproductive potential: the cryopreservation of a testicular cell suspension (TCS) or the cryopreservation of testicular tissue (TET)?DESIGN: Two cryopreservation strategies for spermatogonial stem cells (SSCs) were compared in a mouse model: cryopreservation as TET or as TCS. Evaluated outcomes were number of viable cells after thawing, number and length of donor-derived colonies after spermatogonial stem cell transplantation (SSCT), number of litters, litter size and number of donor-derived pups after mating.RESULTS: Compared with cryopreserving TCS, cryopreservation of TET resulted in significantly higher numbers of viable cells after thawing (TET: 13.4  ×  104 ± 7.2  ×  104 versus TCS: 8.2  ×  104 ± 2.7  ×  104; P = 0.0002), more (TET: 47.6 ± 19.2 versus TCS: 18.5 ± 13.0; P = 0.0039) and longer (TET: 5.2 ± 1.0 mm versus TCS: 2.7 ± 1.5 mm; P = 0.0016) donor-derived colonies, and more donor-derived pups per litter (TET: 2.2 ± 0.2 versus TCS: 0.5 ± 0.1; P = 0.0008).CONCLUSIONS: Cryopreservation of TET is the preferred method to cryopreserve SSCs prior to SSCT in a mouse model.

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Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Abstract

Bibliographical note

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EU470: GROWSPERM: Development of in vitro and in vivo strategies to avoid and cure male infertility.

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Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation

Abstract

Bibliographical note

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Other files and links

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Projects

EU470: GROWSPERM: Development of in vitro and in vivo strategies to avoid and cure male infertility.

Cite this