Tissue clearing for confocal imaging of native and bio-artificial skeletal muscle

L Decroix, V Van Muylder, L Desender, M Sampaolesi, L Thorrez

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Novel clearing techniques have revolutionized three-dimensional confocal imaging of the brain without the need for physical tissue sectioning. We evaluated three clearing methods, ScaleA2, Clear(T2), and 3DISCO for visualizing native and tissue engineered muscle by confocal microscopy. We found that Clear(T2) treatment improved the depth of visualization of immunohistochemical staining slightly, but did not improve depth of visualization of endogenous green fluorescent protein (GFP). ScaleA2 preserved endogenous GFP signal better and permitted significantly deeper GFP imaging, but it was incompatible with tropomyosin immunohistochemical staining. 3DISCO treatment preserved both endogenous GFP and immunohistochemical staining, and permitted significantly deeper imaging. Clearing time for the 3DISCO procedure is short compared to ScaleA2 and Clear(T2). We suggest that 3DISCO is the preferable clearing method for native and tissue engineered skeletal muscle tissue.

    Original languageEnglish
    Pages (from-to)424-431
    Number of pages8
    JournalBiotechnic & Histochemistry
    Volume90
    Issue number6
    DOIs
    Publication statusPublished - 2015

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