Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates

Joske Ruytinx, Adrian R Craciun, Karen Verstraelen, Jaco Vangronsveld, Jan V Colpaert, Nathalie Verbruggen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalMycorrhiza
Volume21
Issue number3
DOIs
Publication statusPublished - Apr 2011

Keywords

  • Amplified Fragment Length Polymorphism Analysis
  • Basidiomycota/genetics
  • Cadmium/metabolism
  • DNA, Complementary/genetics
  • Fungal Proteins/genetics
  • Gene Expression Profiling
  • Molecular Sequence Data
  • Mycorrhizae/genetics
  • Polymorphism, Restriction Fragment Length
  • Soil Microbiology
  • Soil Pollutants/metabolism

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