Tyramine-amplified immunohistochemical testing using "homemade" biotinylated tyramine is highly sensitive and cost-effective

W Jacobs, K Dhaene, E Van Marck

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Immunohistochemical testing using biotinylated tyramine is a recently described technique that results in a strong amplification of the detection signal using antibodies at very high dilutions. In this report we compare the sensitivity and cost-effectiveness of the technique using "homemade" biotinylated tyramine with those of two commercially available kits. The technique using homemade tyramine was easy to follow and highly sensitive and resulted in a 2.5- to 5-fold reduction in costs compared with the commercial kits. Tyramine-amplified immunohistochemical testing using homemade biotinylated tyramine is a valuable and inexpensive tool for both the diagnostic and experimental histopathology laboratory.

Original languageEnglish
Pages (from-to)642-643
Number of pages2
JournalArchives of pathology & laboratory medicine
Volume122
Issue number7
Publication statusPublished - Jul 1998

Keywords

  • Biotinylation
  • Cost-Benefit Analysis
  • Humans
  • Immunoenzyme Techniques/economics
  • Sensitivity and Specificity
  • Tyramine

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