TY - JOUR
T1 - UPLC-PDA-ESI-QTOF-MS/MS and GC-MS analysis of Iranian Dracocephalum moldavica L
AU - Fattahi, Azin
AU - Shakeri, Abolfazl
AU - Tayarani-Najaran, Zahra
AU - Kharbach, Mourad
AU - Segers, Karen
AU - Heyden, Yvan Vander
AU - Taghizadeh, Seyedeh Faezeh
AU - Rahmani, Hanieh
AU - Asili, Javad
N1 - © 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.
PY - 2021/8
Y1 - 2021/8
N2 - Dracocephalum moldavica L. is a significant component in the Iranian food basket. This study aimed to investigate the bioactive compounds and biological activities of different extracts obtained from D. moldavica aerial parts. From the aerial parts, a crude methanolic (MeOH) extract and its four sub-fractions, that is, petroleum ether (Pet), ethyl acetate (EtOAc), n-butanol (n-BuOH), and aqueous (water) extracts were obtained. The total phenolic and flavonoid contents as well as the antioxidant and cytotoxic activities of the extracts were determined. Moreover, the phytochemical profiles of the essential oil (EO) and of those extracts with the highest antioxidant activity measured by GC/MS and UPLC-PDA-ESI-QTOF-MS/MS. Results showed that the highest concentrations of phenols and flavonoids as well as the most potent antioxidant potential according to the DPPH method were determined in the EtOAc and MeOH extracts with IC50 values of 22.0 and 34.4 µg.ml-1, respectively. Quantitative analysis of these extracts was subsequently performed by UPLC-PDA-ESI-QTOF-MS/MS. Both extracts contained mainly rosmarinic acid, caffeic acid, and 2-hydroxycinnamic acid, which may be responsible for their high antioxidant activity. Moreover, none of the extracts showed cytotoxic effects against MCF7, SW48, and a normal cell line of mouse embryonic fibroblast cells (NIH/3T3) in the tested concentrations (up to 400 μg.ml-1). Additionally, GC-MS analysis showed that oxygenated monoterpenes (55.4%) were the main constituents of the EO of D. moldavica.
AB - Dracocephalum moldavica L. is a significant component in the Iranian food basket. This study aimed to investigate the bioactive compounds and biological activities of different extracts obtained from D. moldavica aerial parts. From the aerial parts, a crude methanolic (MeOH) extract and its four sub-fractions, that is, petroleum ether (Pet), ethyl acetate (EtOAc), n-butanol (n-BuOH), and aqueous (water) extracts were obtained. The total phenolic and flavonoid contents as well as the antioxidant and cytotoxic activities of the extracts were determined. Moreover, the phytochemical profiles of the essential oil (EO) and of those extracts with the highest antioxidant activity measured by GC/MS and UPLC-PDA-ESI-QTOF-MS/MS. Results showed that the highest concentrations of phenols and flavonoids as well as the most potent antioxidant potential according to the DPPH method were determined in the EtOAc and MeOH extracts with IC50 values of 22.0 and 34.4 µg.ml-1, respectively. Quantitative analysis of these extracts was subsequently performed by UPLC-PDA-ESI-QTOF-MS/MS. Both extracts contained mainly rosmarinic acid, caffeic acid, and 2-hydroxycinnamic acid, which may be responsible for their high antioxidant activity. Moreover, none of the extracts showed cytotoxic effects against MCF7, SW48, and a normal cell line of mouse embryonic fibroblast cells (NIH/3T3) in the tested concentrations (up to 400 μg.ml-1). Additionally, GC-MS analysis showed that oxygenated monoterpenes (55.4%) were the main constituents of the EO of D. moldavica.
UR - http://www.scopus.com/inward/record.url?scp=85107788208&partnerID=8YFLogxK
U2 - 10.1002/fsn3.2396
DO - 10.1002/fsn3.2396
M3 - Article
C2 - 34401078
VL - 9
SP - 4278
EP - 4286
JO - Food Science and Nutrition
JF - Food Science and Nutrition
SN - 2048-7177
IS - 8
ER -