TY - JOUR
T1 - Xenotransplantation of goat ovary as an alternative to analyse follicles after vitrification
AU - Jiatsa Donfack, Nathalie
AU - Alves, Kele Amaral
AU - Alves, Benner Geraldo
AU - Pedrosa Rocha, Rebeca Magalhães
AU - Bruno, Jamily Bezerra
AU - Lobo, Carlos H
AU - Bertolini, Marcelo
AU - Dos Santos, Regiane Rodrigues
AU - Taumaturgo, Marilia de Oliveira
AU - Raposo, Ramon da Silva
AU - de Figueiredo, José Ricardo
AU - Smitz, Johan
AU - Ribeiro Rodrigues, Ana Paula
N1 - © 2018 Blackwell Verlag GmbH.
PY - 2019/2
Y1 - 2019/2
N2 - The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant. However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant. Follicular density significantly decreased in all treatments in comparison with fresh control. We observed high fibrosis in both fresh transplant and vitrified transplant. The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control. In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft.
AB - The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant. However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant. Follicular density significantly decreased in all treatments in comparison with fresh control. We observed high fibrosis in both fresh transplant and vitrified transplant. The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control. In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft.
KW - folliculogenesis
KW - graft
KW - ovarian cryopreservation
KW - ovarian function restoration
U2 - 10.1111/rda.13340
DO - 10.1111/rda.13340
M3 - Article
C2 - 30203872
VL - 54
SP - 216
EP - 224
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
SN - 0936-6768
IS - 2
ER -