Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Anne-Céline Kohler; Leonardo Venturelli; Abhilash Kannan; Dominique Sanglard; Giovanni Dietler; Ronnie Willaert; Sandor Kasas

doi:10.3390/fermentation6010028

Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Anne-Céline Kohler, Leonardo Venturelli, Abhilash Kannan, Dominique Sanglard, Giovanni Dietler, Ronnie Willaert, Sandor Kasas

Department of Bio-engineering Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.

Original language	English
Article number	28
Journal	Fermentation
Volume	6
Issue number	1
DOIs	https://doi.org/10.3390/fermentation6010028
Publication status	Published - Mar 2020

Bibliographical note

Funding Information:
This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001NCLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor.

Funding Information:
Acknowledgments: The authors thanks C. d\u2019Enfert for providing the Candida albicans strains and S. Leibundgut for highly constructive discussions. The Belgian Federal Science Policy Office (Belspo) and the European Space Agency (ESA) PRODEX program supported this work. The Research Council of the Vrije Universiteit Brussel (Belgium) and the University of Ghent (Belgium) are acknowledged to support the Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), and the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO).

Funding Information:
Funding: This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001N-CLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor.

Publisher Copyright:
© 2020 by the authors.

Keywords

Candida albicans
adhesion
atomic force microscope (AFM)
fibronectin
nanomotion

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title = "Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans",

abstract = "Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.",

keywords = "Candida albicans, adhesion, atomic force microscope (AFM), fibronectin, nanomotion",

author = "Anne-C{\'e}line Kohler and Leonardo Venturelli and Abhilash Kannan and Dominique Sanglard and Giovanni Dietler and Ronnie Willaert and Sandor Kasas",

note = "Funding Information: This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001NCLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor. Funding Information: Acknowledgments: The authors thanks C. d\u2019Enfert for providing the Candida albicans strains and S. Leibundgut for highly constructive discussions. The Belgian Federal Science Policy Office (Belspo) and the European Space Agency (ESA) PRODEX program supported this work. The Research Council of the Vrije Universiteit Brussel (Belgium) and the University of Ghent (Belgium) are acknowledged to support the Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), and the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO). Funding Information: Funding: This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001N-CLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor. Publisher Copyright: {\textcopyright} 2020 by the authors.",

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doi = "10.3390/fermentation6010028",

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AU - Kohler, Anne-Céline

AU - Venturelli, Leonardo

AU - Kannan, Abhilash

AU - Sanglard, Dominique

AU - Dietler, Giovanni

AU - Willaert, Ronnie

AU - Kasas, Sandor

N1 - Funding Information: This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001NCLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor. Funding Information: Acknowledgments: The authors thanks C. d\u2019Enfert for providing the Candida albicans strains and S. Leibundgut for highly constructive discussions. The Belgian Federal Science Policy Office (Belspo) and the European Space Agency (ESA) PRODEX program supported this work. The Research Council of the Vrije Universiteit Brussel (Belgium) and the University of Ghent (Belgium) are acknowledged to support the Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), and the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO). Funding Information: Funding: This research was funded by Schweizerischer Nationalfonds zur F\u00F6rderung der Wissenschaftlichen Forschung 200021-144321 and 407240_167137, the Gebert R\u00FCf Stiftung GRS-024/14, NASA NNH16ZDA001N-CLDTCH, EPFL and ESA PRODEX project Yeast Bioreactor. Publisher Copyright: © 2020 by the authors.

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N2 - Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.

AB - Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.

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JO - Fermentation

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ER -

Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Abstract

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