Identification and characterization of new messenger RNA interferases of Escherichia coli

  • Mikkel Christensen-Dalsgaard ((PhD) Student)
  • Kenn Gerdes (Promotor)
  • Nikolay Zenkin (Jury)
  • Remy Loris (Jury)

Student thesis: Doctoral Thesis

Abstract

Toxin-antitoxin (TA) loci, which are widely distributed in prokaryotes, are organized in small operons, consisting of genes encoding toxins and antitoxins. In this thesis, it is shown that the messenger interferase (mI) YoeB, like RelE, cleaves mRNA strictly dependent on translation in vivo but that mRNA cleavage by MazF occurs independent of translation. Expression of Doc from phage P1 medites mRNA cleavage indirectly through activation of endogenous E. coli RelE. IN a second part of the thesis, three novel mI's from E. coli were characterized and shown to be differentially induced by different stresses. Finally, a novel synthetic lethal screen was developed to look for synthetic letal or sic interactions with tmRNA, the molecule required for trans-tranlation and part of the recovery mechanism or mI's.
Date of Award8 Jul 2011
Original languageEnglish
SupervisorRemy Loris (Jury), Kenn Gerdes (Promotor) & Nikolay Zenkin (Jury)

Keywords

  • Toxin-antitoxin modules
  • trans-translation
  • bacterial stress response

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