Pan cancer single-cell atlas of mouse and human tumor-associated dendritic cells

Activiteit: Talk or presentation at a conference

Description

Dendritic cells (DCs) are known to be key in orchestrating anti-tumor immunity. However, multiple studies have described several DC subsets/substates in inflammatory settings and homeostasis, however often using non-consistent nomenclature, complicating the comparison and translation across studies. Aiming to bridge this knowledge gap we generated respective mouse and human tumor-associated DC (TADC) scRNA-seq atlases. The mouse atlas comprises Lewis Lung Carcinoma (LLC, LLC-OVA), B16 melanoma, E0771 breast cancer, and GL261 glioblastoma, while the human atlas constitutes 10 different cancer types.
In both atlases, we identified known DC subsets/substates including cDC1, cDC2a, Inf-DC, DC3, CCR7+ DCs, and DC precursors. We highlight the correlation between mouse and human TADC subsets across different cancers and gene signatures common to both species. Moreover, we compare the TADC subsets with bone marrow and spleen DC subsets from naïve and tumor-bearing mice to assess the influence of cancer-induced inflammation on TADC subsets. This also gives insight into differences in DC phenotypes across different tissues in naïve versus tumor-bearing mice. Furthermore, we characterize phenotypic and predicted functional differences in CCR7+ DCs resident in the tumor versus those found in the tumor-draining and naïve lymph nodes. Additionally, we assess the correlation between different DC subsets in human cancers and with disease prognosis. Finally, using spatial transcriptomics we assessed where different DC subsets lie within mouse tumors, particularly with respect to hypoxia.
In conclusion, we generated mouse and human TADC atlases, which are valuable tools for characterizing the pan-cancer DC landscape across tumors, tumor-associated, and naive secondary lymphoid organs. A comprehensive understanding of TADCs across different cancers could give insights into potential DC-targeted anti-cancer strategies in the future.
Periode9 jun. 2024
Evenementstitel19th International Conference on Innate Immunity
EvenementstypeConference
LocatieLoutraki, GreeceToon op kaart
Mate van erkenningInternational