Printing of bone cells on micropatterned networks to study osteoblast-osteocyte communication

Yvanoff, C. (Speaker), Willaert, R. (Contributor)

Activiteit: Talk or presentation at a workshop/seminar

Description

Bone tissue homeostasis relies on the balanced activities of two cell types: the bone-forming osteoblasts and the bone-degrading osteoclasts. This equilibrium is ensured by the last bone cell type, namely the osteocyte. Over the past 30 years, it has been extensively reported that bone cells are sensitive and responsive to mechanical stimulation. More precisely, mechanical cues promote bone strength whereas the lack of physical stimuli results in significant bone loss. However, despite many in vivo and in vitro studies, the molecular mechanisms through which bone cells communicate together to maintain bone tissue homeostasis in response to mechanostimulation are hardly understood.
In light of the above, our research aims at contributing to the understanding of the molecular bases underlying bone cell mechanobiology. Specifically, we focus on developing a platform to study cell-cell interactions of bone cells under mechanostimulation. To do so, we use state-of-the-art technologies and combine for the first time robotic cell printing with cell micropatterning. Cell micropatterning is used to generate physiological-like networks of interacting bone cells (osteoblasts and osteocytes), whereas robotic printing is used to deliver the bone cells to their respective areas on the micropatterned network. The resulting platform overcomes the randomness of conventional methods (co-cultures, parachute assays,…) by creating controlled osteoblast-osteocyte interactions. Finally, the platform is covered with the top of a microfluidic channel, which enables us to perform mechanostimulation experiments by applying a fluid flow shear stress.
Periode30 sep 20191 okt 2019
EvenementstitelSpot your future – MMM 2019
EvenementstypeWorkshop
LocatieVienna, Austria
Mate van erkenningInternational