Projecten per jaar
Persoonlijk profiel
Expertise
BIO: Janine Brunner is since 2020 group leader at the VIB-VUB Center for Structural Biology leading the lab Structure and Function of Membrane Proteins and professor at the Vrije Universiteit Brussel. She obtained her PhD in 2015 from the University of Zurich/Switzerland with structural and functional work on phospholipid scramblases in the TMEM16 family. It provided the first atomic insight into the architecture and working mechanism of Ca2+ activated phospholipid scramblases and contributed significant new concepts in this field. During postdoc stays at Universities of Zurich and Basel and the Paul-Scherrer Institute (ETH)/Switzerland she studied non-canonical lysosomal potassium channels that are associated with Parkinson’s disease by combining X-ray crystallography with electrophysiology.Her results have revealed how ion selectivity and gating is achieved in these newly discovered potassium channels.
Janine’s efforts advanced the understanding in several membrane protein classes and her work, published in Nature, Nature Communications, eLife, and Current Opinion in Structural Biology, has been referenced more than 460 times (source scopus). She was invited twice to give a talk at the New and Notable session, prestigious platforms at the Biophysics Society Annual Meeting and the European Biophysics Congress.
MISSION: The Brunner lab (started in 2020) is focusing on the structural biology of membrane proteins, and their functional characterization. With the major topic of membrane proteins in lipid transport and metabolism and their significance for membrane asymmetry we engage in a central theme of cell biology and aim to answer long-standing questions in lipid trafficking and signalling. The second theme covers structural biology and biophysics of ion channels implicated in lysosome physiology and pathological instances such as Parkinson’s disease. Her group is capitalizing from extensive experience in membrane protein biochemistry and structural biology and integrates additional methodologies such as patch-clamp electrophysiology and fluorescence microscopy to achieve a comprehensive understanding of the research subjects.
For more information: https://brunnerlab.sites.vib.be/en
Externe posities
Postdoc in the lab of Michel Steinmetz, Paul Scherrer Institute
jan 2018 → nov 2019Postdoc in the lab of Timm Maier, University of Basel
jan 2017 → dec 2017Postdoc in the lab of Raimund Dutzler, University of Zurich
2015 → 2016PhD thesis in the Lab or Raimund Dutzler, University of Zurich
2010 → 2015Vingerafdruk
- 1 Soortgelijke profielen
Netwerk
Projecten
- 4 Actief
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HERC58: SPECY-SORT: SPEctrale flowCYtometer en celSORTer
Laoui, D., De Veirman, K., Van der Henst, C., Brunner, J., Messens, J., Raes, G., Van Ginderachter, J., Devoogdt, N., Magez, S., Remaut, H. K., Stijlemans, B., Movahedi, K., Peeters, E. & De Trez, C.
1/05/22 → 30/04/26
Project: Fundamenteel
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OZR3941: Cofinanciering OZR FWO Hercules MZW: SPECY-SORT: SPEctrale flowCYtometer en celSORTer
Laoui, D., Van Ginderachter, J., Movahedi, K., De Veirman, K., Magez, S., De Trez, C., Stijlemans, B., Raes, G., Devoogdt, N., Messens, J., Brunner, J., Remaut, H. K., Fioravanti, A., Peeters, E. & Van der Henst, C.
16/03/22 → 16/03/25
Project: Fundamenteel
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Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation
Botte, M., Ni, D., Schenck, S., Zimmermann, I., Chami, M., Bocquet, N., Egloff, P., Bucher, D., Trabuco, M., Cheng, R. K. Y., Brunner, J., Seeger, M. A., Stahlberg, H. & Hennig, M., dec 2022, In: Nature Communications. 13, 1, 1826.Onderzoeksoutput: Article › peer review
Open AccessBestand3 Citaten (Scopus)20 Downloads (Pure) -
Insight into Lipopolysaccharide Translocation by Cryo-EM structures of a LptDE Transporter in Complex with Pro-Macrobodies
Botte, M., Ni, D., Schenck, S., Zimmermann, I., Chami, M., Bocquet, N., Egloff, P., Bucher, D., Trabuco, M., Cheng, R. K. Y., Brunner, J., Seeger, M. A., Stahlberg, H. & Hennig, M., 23 mrt 2021, (BIORXIV).Onderzoeksoutput: Preprint
Open Access -
Production and Application of Nanobodies for Membrane Protein Structural Biology
Brunner, J. D. & Schenck, S., feb 2020, Production and Application of Nanobodies for Membrane Protein Structural Biology. Maier, T. & Perez, C. (redactie). Humana Press, Vol. 2127. blz. 167-184 18 blz.Onderzoeksoutput: Chapter › Research › peer review
2 Citaten (Scopus) -
Structural basis for ion selectivity in TMEM175 K+ channels
Brunner, J. D., Jakob, R. P., Schulze, T., Neldner, Y., Moroni, A., Thiel, G., Maier, T. & Schenck, S., 8 apr 2020, In: eLife. 9, 9, e53683.Onderzoeksoutput: Article › peer review
Open AccessBestand16 Citaten (Scopus)139 Downloads (Pure) -
Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity
Brunner, J. D. & Schenck, S., 2019, Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity . Drin, G. (redactie). Intracellular lipid transport redactie Humana Press, Vol. 1949. blz. 181-199Onderzoeksoutput: Chapter › Research › peer review
3 Citaten (Scopus)
Prijzen
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Best PhD thesis of the year (2015), by the University of Zürich, Switzerland
Brunner, Janine (Recipient), 2015
Prijs: National/international honour
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Invited speaker for New and Notable Session Talk at the 10th European Biophysics Congress (2015) Dresden, Germany
Brunner, Janine (Recipient), 2015
Prijs: National/international honour
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New and Notable Session Talk at the Biophysical Society 59th Annual Meeting in Baltimore, USA
Brunner, Janine (Recipient), 2015
Prijs: National/international honour
Activiteiten
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Toegepaste Biologische Wetenschappen (Organisatie)
Janine Brunner (Jury)
1 jan 2023Activiteit: Membership of peer-review panel or committee
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Toegepaste Biologische Wetenschappen (Organisatie)
Janine Brunner (Member)
8 jun 2022Activiteit: Membership of peer-review panel or committee
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Toegepaste Biologische Wetenschappen (Organisatie)
Janine Brunner (Member)
4 jul 2022Activiteit: Membership of peer-review panel or committee
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Toegepaste Biologische Wetenschappen (Organisatie)
Janine Brunner (Member)
16 dec 2022Activiteit: Membership of peer-review panel or committee
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Toegepaste Biologische Wetenschappen (Organisatie)
Eveline Peeters (Chair), Janine Brunner (Jury), Ilse Smolders (Jury), Steffen Jung (Jury), Diego Gomez-Nicola (Jury), Kiavash Movahedi (Supervisor) & Jo Van Ginderachter (Supervisor)
8 jun 2022Activiteit: Member of PhD committee
Scriptie
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Recombinant expression, purification and initial characterization of human BBS2+9
Auteur: Hoenjet, E., Loris, R., Muruganandam, G., Brunner, J. & Leyns, L., 7 sep 2021Scriptie/Masterproef: Master's Thesis
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Unraveling the structure and function of the synaptic proteins TBC1D24 and Synaptojanin1 and their role in neurological disorders
Auteur: Paesmans, J., Versées, W., Ballet, S., Brunner, J., Smolders, I., Greggio, E. & Ismail, S., 29 jun 2021Scriptie/Masterproef: Doctoral Thesis