1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family

Maruša Prolič Kalinšek; Pieter De Bruyn; Dukas Jurenas; Laurence Van Melderen; Remy Loris; A.N. Volkov

doi:10.1007/s12104-019-09915-9

1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family

Maruša Prolič Kalinšek, Pieter De Bruyn, Dukas Jurenas, Laurence Van Melderen, Remy Loris, A.N. Volkov

Onderzoeksoutput: Article › peer review

2 Citaten (Scopus)

Samenvatting

The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the 1H, 15N and 13C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.

Originele taal-2	English
Pagina's (van-tot)	25-30
Aantal pagina's	6
Tijdschrift	Biomolecular NMR Assignments
Volume	14
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1007/s12104-019-09915-9
Status	Published - apr. 2020

Bibliografische nota

Funding Information:
This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13).

Funding Information:
This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13).

Publisher Copyright:
© 2019, Springer Nature B.V.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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10.1007/s12104-019-09915-9

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FWOAL831: Regulatie van persistentie door het paaAR-paaA2-parE2 en cog4197-duf1019 operons van E. coli O157
Loris, R. (Administrative Promotor)
1/01/17 → 31/12/20
Project: Fundamenteel
SRP13: Structuur en dynamiek van macromoleculaire complexen in gezondheid en ziekte
Steyaert, J. (Administrative Promotor), Remaut, H. K. (Co-Promoter), Loris, R. (Co-Promoter), Efremov, R. (Co-Promoter), Steyaert, J. (Administrative Promotor), Loris, R. (Co-Promoter) & Remaut, H. K. (Co-Promoter)
1/11/12 → 31/10/24
Project: Fundamenteel

2 Citaties
2 Article
1 PhD Thesis

Bistable expression of a toxin-antitoxin system located in a cryptic prophage of Escherichia coliO157:H7
Jurenas, D., Fraikin, N., Goormaghtigh, F., De Bruyn, P., Vandervelde, A., Jove, T., Charlier, D., Loris, R. & Van Melderen, L., 21 dec. 2021, In: mBio. 12, 6, 15 blz., e0294721.
Onderzoeksoutput: Article › peer review

Open Access
Bestand
16 Citaten (Scopus)

254 Downloads (Pure)
Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7
De Bruyn, P., Prolic Kalinsek, M., Vandervelde, A., Malfait, M., Sterckx, Y., Sobott, F., Hadzi, S., Pardon, E., Steyaert, J. & Loris, R., 1 okt. 2021, In: Acta Crystallographica Section F - Structural Biology Communications. 77, 10, blz. 374-384 11 blz., 11.
Onderzoeksoutput: Article › peer review

Open Access
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1 Citaat (Scopus)

310 Downloads (Pure)
Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria
De Bruyn, P., 2021, Brussels.
Onderzoeksoutput: PhD Thesis

PhD Defense Mariusa Prolic-Kalinsek - Regulatory interplay between the toxin-antitoxin cluster paaR2-paaA2-parE2 and prophage genes ydaS-ydaT from Escherichia coliO157:H7 (Evenement)
Stijlemans, B. (Jury), Prolic Kalinsek, M. (Speaker), Tompa, P. (Chair), Brunner, J. (Jury), De Proft, F. (Jury), Strelkov, S. (Jury), Bouckaert, J. (Jury) & Loris, R. (Supervisor)
16 mei 2023
Activiteit: Member of PhD committee
PhD Thesis defense Pieter De Bruyn - Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria (Evenement)
Peeters, E. (Chair), Van Molle, I. (Jury), Roelants, K. (Jury), Loris, R. (Supervisor), De Greve, H. (Supervisor), Tenson, T. (Jury) & Michiels, J. (Jury)
2 sep. 2021
Activiteit: Member of PhD committee

Citeer dit

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abstract = "The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the 1H, 15N and 13C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.",

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note = "Funding Information: This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13). Funding Information: This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13). Publisher Copyright: {\textcopyright} 2019, Springer Nature B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1007/s12104-019-09915-9",

language = "English",

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AU - Prolič Kalinšek, Maruša

AU - De Bruyn, Pieter

AU - Jurenas, Dukas

AU - Van Melderen, Laurence

AU - Loris, Remy

AU - Volkov, A.N.

N1 - Funding Information: This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13). Funding Information: This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13). Publisher Copyright: © 2019, Springer Nature B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4

Y1 - 2020/4

N2 - The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the 1H, 15N and 13C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.

AB - The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the 1H, 15N and 13C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.

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1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family

Samenvatting

Bibliografische nota

Toegang tot document

Handle.net

Andere bestanden en links

Vingerafdruk

Projecten

FWOAL831: Regulatie van persistentie door het paaAR-paaA2-parE2 en cog4197-duf1019 operons van E. coli O157

SRP13: Structuur en dynamiek van macromoleculaire complexen in gezondheid en ziekte

Onderzoekersoutput

Bistable expression of a toxin-antitoxin system located in a cryptic prophage of Escherichia coliO157:H7

Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7

Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria

Activiteiten

PhD Defense Mariusa Prolic-Kalinsek - Regulatory interplay between the toxin-antitoxin cluster paaR2-paaA2-parE2 and prophage genes ydaS-ydaT from Escherichia coliO157:H7 (Evenement)

PhD Thesis defense Pieter De Bruyn - Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria (Evenement)

Citeer dit