A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages

Jung-Seok Kim, Masha Kolesnikov, Shany Peled-Hajaj, Isabelle Scheyltjens, Yuan Xia, Sebastien Trzebanski, Zhana Haimon, Anat Shemer, Alisa Lubart, Hannah Van Hove, Louise Chappell-Maor, Sigalit Boura-Halfon, Kiavash Movahedi, Pablo Blinder, Steffen Jung

Onderzoeksoutput: Articlepeer review

69 Citaten (Scopus)


The developmental and molecular heterogeneity of tissue macrophages is unravelling, as are their diverse contributions to physiology and pathophysiology. Moreover, also given tissues harbor macrophages in discrete anatomic locations. Functional contributions of specific cell populations can in mice be dissected using Cre recombinase-mediated mutagenesis. However, single promoter-based Cre models show limited specificity for cell types. Focusing on macrophages in the brain, we establish here a binary transgenic system involving complementation-competent NCre and CCre fragments whose expression is driven by distinct promoters: Sall1ncre: Cx3cr1ccre mice specifically target parenchymal microglia and compound transgenic Lyve1ncre: Cx3cr1ccre animals target vasculature-associated macrophages, in the brain, as well as other tissues. We imaged the respective cell populations and retrieved their specific translatomes using the RiboTag in order to define them and analyze their differential responses to a challenge. Collectively, we establish the value of binary transgenesis to dissect tissue macrophage compartments and their functions.

Originele taal-2English
Pagina's (van-tot)176-190
Aantal pagina's15
Nummer van het tijdschrift1
Vroegere onlinedatum11 dec 2020
StatusPublished - 12 jan 2021

Bibliografische nota

Copyright © 2020. Published by Elsevier Inc.


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