Pancreatic acinar cells secrete digestive enzymes necessary for nutrient digestion in the intestine. They are considered the initiating cell type of pancreatic cancer and are endowed with differen plasticity that has been harnessed to regenerate endocrine beta cells. However, there is still uncertainty about the mechanisms of acinar cell formation during the dynamic period of early po development. To unravel cellular contributions in the exocrine acinar development we studied two reporter mouse strains to trace the fate of acinar and duct cells during the first 4 weeks of life received tamoxifen on the day of birth and were followed during the next four weeks. In the acinar reporter mice ElastaseCreER R26-YFP, the labelling index of acinar cells remained unchanged the neonatal pancreas growth period (35.3 ± 5.4% YFP+ acinar cells (n = 7) at 1-week of age vs 35.4 ± 5.6% YFP+ acinar cells at week 4 (n = 10) (p > 0.05)), evidencing that acinar cells are formed duplication. In line with this, duct cell tracing in Hnf1bCreER R26-LacZ mice did not show significant increase in acinar cell labelling (0.02 ± 0.02% X-gal+ acinar cells at 4-weeks of age (n = 4)), exc duct-to-acinar cell contribution during neonatal development. Immunohistochemical analysis confirms massive levels of acinar cell proliferation in this early period of life, as more than 40% of am cells are positive for Ki67 at week 1. Further, also increase in acinar cell size contributes to the growth of pancreatic mass. Moreover, the mean number of acinar nuclei per acinus remains co throughout neonatal development. We conclude that the growth of acinar cells during physiological neonatal pancreas development is by self-duplication (and hypertrophy) rather than neog from progenitor cells as was suggested before.
|Titel||• Poster presentation at Annual meeting of the Belgian Society for Stem Cell Research, Leuven, Belgium|
|Status||Published - 26 okt 2018|
|Evenement||Annual meeting of the Belgian Society for Stem Cell Research - Leuven, Belgium|
Duur: 26 okt 2018 → 26 okt 2018
|Conference||Annual meeting of the Belgian Society for Stem Cell Research|
|Periode||26/10/18 → 26/10/18|