Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres

Karen Sermon; Willy Lissens; Paul Devroey; Andre Van Steirteghem; Ingeborg Liebaers

Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres

Karen Sermon, Willy Lissens, Paul Devroey, Andre Van Steirteghem, Ingeborg Liebaers

Onderzoeksoutput: Article › peer review

12 Citaten (Scopus)

Samenvatting

OBJECTIVE: To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease. DESIGN: Polymerase chain reaction on single human blastomeres. SETTING: Bad quality embryos from IVF analyzed in an academic research genetics lab. INTERVENTIONS: Patients underwent standard IVF procedures as infertility treatment. RESULTS: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. CONCLUSION: The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.

Originele taal-2	English
Pagina's (van-tot)	407-409
Aantal pagina's	3
Tijdschrift	Fertil Steril
Volume	63
Nummer van het tijdschrift	February
Status	Published - 1995

Bibliografische nota

Fertil Steril 63, 407-409, 1995.

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title = "Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres",

abstract = "OBJECTIVE: To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease. DESIGN: Polymerase chain reaction on single human blastomeres. SETTING: Bad quality embryos from IVF analyzed in an academic research genetics lab. INTERVENTIONS: Patients underwent standard IVF procedures as infertility treatment. RESULTS: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. CONCLUSION: The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.",

keywords = "PGD, Tay-Sachs disease",

author = "Karen Sermon and Willy Lissens and Paul Devroey and {Van Steirteghem}, Andre and Ingeborg Liebaers",

note = "Fertil Steril 63, 407-409, 1995.",

year = "1995",

language = "English",

volume = "63",

pages = "407--409",

journal = "Fertility and Sterility",

issn = "0015-0282",

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number = "February",

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TY - JOUR

T1 - Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres

AU - Sermon, Karen

AU - Lissens, Willy

AU - Devroey, Paul

AU - Van Steirteghem, Andre

AU - Liebaers, Ingeborg

N1 - Fertil Steril 63, 407-409, 1995.

PY - 1995

Y1 - 1995

N2 - OBJECTIVE: To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease. DESIGN: Polymerase chain reaction on single human blastomeres. SETTING: Bad quality embryos from IVF analyzed in an academic research genetics lab. INTERVENTIONS: Patients underwent standard IVF procedures as infertility treatment. RESULTS: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. CONCLUSION: The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.

AB - OBJECTIVE: To evaluate the possibility of using polymerase chain reaction (PCR) technology for preimplantation diagnosis for Tay-Sachs disease. DESIGN: Polymerase chain reaction on single human blastomeres. SETTING: Bad quality embryos from IVF analyzed in an academic research genetics lab. INTERVENTIONS: Patients underwent standard IVF procedures as infertility treatment. RESULTS: Amplification was seen in 89.5% of the blastomeres; only one blank was contaminated. CONCLUSION: The technique of PCR on single blastomeres is ready to be used in clinical preimplantation diagnosis for Tay-Sachs disease.

KW - PGD

KW - Tay-Sachs disease

M3 - Article

VL - 63

SP - 407

EP - 409

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - February

ER -

Amplification of exon 11 of the gene for the alpha-chain of beta-N-acetylhexosaminidase in single human blastomeres

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Bibliografische nota

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