TY - JOUR
T1 - Antibacterial activity and mutagenesis of sponge-associated Pseudomonas fluorescens H41
AU - Ye, Lumeng
AU - Santos-Gandelman, Juliana F
AU - Hardoim, Cristiane C P
AU - George, Isabelle
AU - Cornelis, Pierre
AU - Laport, Marinella S
PY - 2015/7
Y1 - 2015/7
N2 - Marine sponges (phylum Porifera) are well known to harbour a complex and diverse bacterial community. Some of these sponge-associated bacteria have been shown to be the real producers of secondary metabolites with a wide range of activities from antimicrobials to anticancer agents. Previously, we revealed that the strain Pseudomonas fluorescens H41 isolated from the sponge Haliclona sp. (collected at the coast of Rio de Janeiro, Brazil) showed a strong antimicrobial activity against clinical and marine bacteria. Thus, in this study the genes involved in the antimicrobial activity of P. fluorescens H41 were identified. To this end, a library of mutants was generated via miniTnphoA3 transposon mutagenesis and the resulting clones were characterized for their antimicrobial activity. It was demonstrated that genes involved in the biosynthesis of the pyoverdine siderophore are related to the inhibitory activity of P. fluorescens H41. Therefore, this strain might play an important role in the biocontrol of the host sponge.
AB - Marine sponges (phylum Porifera) are well known to harbour a complex and diverse bacterial community. Some of these sponge-associated bacteria have been shown to be the real producers of secondary metabolites with a wide range of activities from antimicrobials to anticancer agents. Previously, we revealed that the strain Pseudomonas fluorescens H41 isolated from the sponge Haliclona sp. (collected at the coast of Rio de Janeiro, Brazil) showed a strong antimicrobial activity against clinical and marine bacteria. Thus, in this study the genes involved in the antimicrobial activity of P. fluorescens H41 were identified. To this end, a library of mutants was generated via miniTnphoA3 transposon mutagenesis and the resulting clones were characterized for their antimicrobial activity. It was demonstrated that genes involved in the biosynthesis of the pyoverdine siderophore are related to the inhibitory activity of P. fluorescens H41. Therefore, this strain might play an important role in the biocontrol of the host sponge.
KW - Animals
KW - Anti-Bacterial Agents
KW - Brazil
KW - DNA Transposable Elements
KW - Haliclona
KW - Metabolic Networks and Pathways
KW - Mutagenesis, Insertional
KW - Oligopeptides
KW - Pseudomonas fluorescens
KW - Research Support, Non-U.S. Gov't
U2 - 10.1007/s10482-015-0469-4
DO - 10.1007/s10482-015-0469-4
M3 - Article
C2 - 25957971
VL - 108
SP - 117
EP - 126
JO - Antonie Van Leeuwenhoek
JF - Antonie Van Leeuwenhoek
SN - 0003-6072
IS - 1
ER -