Arabidopsis APx-R Is a Plastidial Ascorbate-Independent Peroxidase Regulated by Photomorphogenesis

Fernanda Lazzarotto, Khadija Wahni, Maiara Piovesana, Felipe Maraschin, Joris Messens, Marcia Margis-Pinheiro

Onderzoeksoutput: Articlepeer review

6 Citaten (Scopus)

Samenvatting

Peroxidases are enzymes that catalyze the reduction of hydrogen peroxide, thus minimizing cell injury and modulating signaling pathways as response to this reactive oxygen species. Using a phylogenetic approach, we previously identified a new peroxidase family composed of a small subset of ascorbate peroxidase (APx) homologs with distinguished features, which we named ascorbate peroxidase-related (APx-R). In this study, we showed that APx-R is an ascorbate-independent heme peroxidase. Despite being annotated as a cytosolic protein in public databases, transient expression of AtAPx-R-YFP in Arabidopsis thaliana protoplasts and stable overexpression in plants showed that the protein is targeted to plastids. To characterize APx-R participation in the antioxidant metabolism, we analyzed loss-of-function mutants and AtAPx-R overexpressing lines. Molecular analysis showed that glutathione peroxidase 7 (GPx07) is specifically induced to compensate the absence of APx-R. APx-R overexpressing lines display faster germination rates, further confirming the involvement of APx-R in seed germination. The constitutive overexpression of AtAPx-R-YFP unraveled the existence of a post-translational mechanism that eliminates APx-R from most tissues, in a process coordinated with photomorphogenesis. Our results show a direct role of APx-R during germinative and post-germinative development associated with etioplasts differentiation.

Originele taal-2English
Artikelnummer65
Pagina's (van-tot)1-15
Aantal pagina's15
TijdschriftAntioxidants
Volume10
Nummer van het tijdschrift1
DOI's
StatusPublished - 7 jan 2021

Bibliografische nota

Funding Information:
This work was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a VIB grant, and the Research Foundation-Flanders–Fonds de la Recherche Scientifique Excellence of Science project No. 30829584. F.L. was supported with the Wood-Whelan Research Fellowship from the International Union of Biochemistry and Molecular Biology, and by the Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM). This work was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (to M.M.-P.), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (to M.M.-P.), a VIB grant (to J.M.), and the Research Foundation-Flanders–Fonds de la Recherche Scientifique Excellence of Science project No. 30829584 (to J.M.). F.L. was supported with the Wood-Whelan Research Fellowship from the International Union of Biochemistry and Molecular Biology, and by the Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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