@article{d20e5383cd464b2c97e668ca30f069d7,
title = "Biophysical characterization of the Plasmodium falciparum circumsporozoite protein{\textquoteright}s N-terminal domain",
abstract = "The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti-malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N-terminal domain (CSPN) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSPN is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSPN from Plasmodium falciparum (PfCSPN), which provides pure, high-quality protein preparations with high yields. Through an interdisciplinary approach combining in-solution experimental methods and in silico analyses, we provide strong evidence that PfCSPN is an intrinsically disordered region displaying some degree of compaction.",
author = "Rob Geens and Jessica Stanisich and O Beyens and S. D'Hondt and J.M. Thiberge and A. Ryckebosch and {De Groot}, A. and Stefan Magez and Didier Vertommen and R. Amino and {De Winter}, H. and A.N. Volkov and Peter Tompa and Y.G.J. Sterckx",
note = "Funding Information: Rob Geens is a doctoral fellow supported by a DOCPRO4-NIEUWZAP (code 40043) grant awarded to Yann G.-J. Sterckx by the University of Antwerp “Bijzonder Onderzoeksfonds (BOF).” Olivier Beyens is a PhD fellow supported by FWO-Vlaanderen (1S04221N). Rogerio Amino is funded by the Agence Nationale de la Recherche (SporoSTOP ANR-19-CE15-0027), and the French Government's Investissement d'Avenir program, Laboratoire d'Excellence “Integrative Biology of Emerging Infectious Diseases” (ANR-10-LABX-62-IBEID). The authors wish to thank the staff of the SWING beam line at SOLEIL synchrotron (Javier Perez and Thomas Bizien) for outstanding beam line support. The authors acknowledge use of the CalcUA and VSC supercomputing facilities and wish to thank the staff for outstanding support. Funding Information: Rob Geens is a doctoral fellow supported by a DOCPRO4‐NIEUWZAP (code 40043) grant awarded to Yann G.‐J. Sterckx by the University of Antwerp “Bijzonder Onderzoeksfonds (BOF).” Olivier Beyens is a PhD fellow supported by FWO‐Vlaanderen (1S04221N). Rogerio Amino is funded by the Agence Nationale de la Recherche (SporoSTOP ANR‐19‐CE15‐0027), and the French Government's Investissement d'Avenir program, Laboratoire d'Excellence “Integrative Biology of Emerging Infectious Diseases” (ANR‐10‐LABX‐62‐IBEID). The authors wish to thank the staff of the SWING beam line at SOLEIL synchrotron (Javier Perez and Thomas Bizien) for outstanding beam line support. The authors acknowledge use of the CalcUA and VSC supercomputing facilities and wish to thank the staff for outstanding support. Publisher Copyright: {\textcopyright} 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.",
year = "2024",
month = jan,
doi = "https://doi.org/10.1002/pro.4852",
language = "English",
volume = "33",
journal = "Protein Science",
issn = "0961-8368",
publisher = "Cold Spring Harbor Laboratory Press",
number = "1",
}