Clinical experience with preimplantation genetic diagnosis and intracytoplasmic sperm injection.

Paul Devroey, ELVIRE VAN ASSCHE, Andre Van Steirteghem, Ingeborg Liebaers, Karen Sermon, Mary-Louise Bonduelle, Hubert Joris, Willy Lissens, Catherine Staessen, Marc Vandervorst, Peter Nagy, Basil Tarlatzis (Redacteur)

Onderzoeksoutput: Articlepeer review

57 Citaten (Scopus)

Samenvatting

Preimplantation genetic diagnosis (PGD) is a novel procedure whose use may be considered to obtain a very early prenatal diagnosis for couples at risk for transmitting genetic diseases. Using the polymerase chain reaction (PCR) or fluorescence in-situ hybridization (FISH) the genotype or the sex of biopsied cleavage-stage embryos obtained after in-vitro fertilization can be determined and selected embryos can then be transferred. In-vitro fertilization with intracytoplasmic sperm injection is the method of choice to obtain embryos to be analysed through PCR to reduce contamination by residual sperm DNA. In our series of 61 PGD cycles for 29 couples at risk over a period of 4 years the ongoing pregnancy rate per cycle was 15%, per transfer 19% and per patient 31%. One of the six morphologically normal children born, who is still alive and doing well, weighed 850 g after birth at 25 weeks following a complicated triplet pregnancy. More experience is needed to correctly evaluate the efficiency and safety of this novel technique as well as to determine its place in the prevention of genetic disease.
Originele taal-2English
Pagina's (van-tot)186-195
Aantal pagina's10
TijdschriftHum Reprod
Volume13
StatusPublished - 1998

Bibliografische nota

In Current Theory and Practice of ICSI. Devroey P, Tarlatzis B, Van Steirteghem A (eds), Oxford, UK, Oxford University Press. Hum Reprod 13, suppl 1, 186-195, 1998.

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