Comparative analysis of vertebrate posterior HoxD clusters: atypical cluster architecture in the caecilian

An Mannaert, Chris Amemiya, Franky Bossuyt

Onderzoeksoutput: Meeting abstract (Book)


The posterior genes of the HoxD cluster play a crucial role in the patterning of the tetrapod limb. During limb development, the posterior HoxD genes are under the control of a global, long-range enhancer that is present in all vertebrates. We sequenced the posterior end of the HoxD cluster (from Evx-2 to Hoxd-10) of a caecilian (a limbless amphibian) in order to perform comparative analyses of this region using other vertebrate HoxD cluster sequences. We determined the presence of evolutionarily conserved noncoding sequences and traced gains and losses of these phylogenetic footprints during vertebrate evolution, while focusing on amphibians, a lineage with a large variety of limb types. At least one and possibly two conserved non-coding sequences were lost in the ancestor of all living amphibians. We also found that the caecilian HoxD cluster is almost three times larger than its mammalian counterpart and that it has experienced the loss of one gene accompanied with an accumulation of inverted and interspersed repeats in that area. A similar event occurred in the coelacanth and snakes. In each case, the cluster enlargement may influence the action of the long-range enhancer on the genes located after the void. Therefore it is possible that body elongation and limb loss in caecilians and snakes are the result of parallel evolutionary changes in the same genetic mechanism.
Originele taal-2English
TitelSMBE Meeting, 2-8 June 2010, Lyon: Poster
StatusPublished - 2010
EvenementUnknown - Stockholm, Sweden
Duur: 21 sep 200925 sep 2009

Publicatie series

NaamSMBE Meeting, 2-8 June 2010, Lyon: Poster




Duik in de onderzoeksthema's van 'Comparative analysis of vertebrate posterior HoxD clusters: atypical cluster architecture in the caecilian'. Samen vormen ze een unieke vingerafdruk.

Citeer dit