Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene.

D. Colau, Ioan Negrutiu, Marc Van Montagu, Jean-Pierre Hernalsteens

Onderzoeksoutput: Articlepeer review

21 Citaten (Scopus)

Samenvatting

The Saccharomyces cerevisiae ILV1 gene, encoding threonine dehydratase (EC 4.2.1.16) was fused to the transferred DNA nopaline synthase promoter and the 3' noncoding region of the octopine synthase gene. It was introduced, by Agrobacterium tumefaciens-mediated gene transfer, into an isoleucine-requiring Nicotiana plumbaginifolia auxotroph deficient in threonine dehydratase. Functional complementation by the ILV1 gene product was demonstrated by the selection of several transformed lines on a medium without isoleucine and by the identification in these lines of the yeast threonine dehydratase activity. This is the first example illustrating the complementation of a plant auxotroph by transfection with a cloned gene.
Originele taal-2English
Pagina's (van-tot)2552-2557
Aantal pagina's6
TijdschriftMolecular and cellular biology
Volume7
StatusPublished - 1987

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