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We report on a numerical simulation study of a number of potential column technology solutions to minimize the plate height contribution (Hvh) originating from the use of ultrahigh pressures and their concomitant viscous heating effect. Looking as far as possible into the future of UHPLC, all main results are obtained for the case of a 2500 bar pressure gradient. However, to generalize the result, a correlation is given that can be used to interpolate the results to lower pressures with some 10% accuracy. For the considered case of a 2.1mm column, a liquid flow rate of 0.45 ml/min, an analyte with retention factor k(25°C)=3 and a retention enthalpy chosen such that ΔHR/R= 1000 K, it is found that, in order to keep the global plate height as measured at the column outlet (Hvh,glob,out) below 1 μm, the bed conductivity would need to be raised to λbed=2.4 W/m•K, i.e., 4 times higher than a typical packed bed of fullyporous or coreshell silica particles. An equivalent effect on the band broadening could be obtained if it would be possible to replace the steel column wall with a low conductivity material. In this case, a wall conductivity of 0.25 W/m•K, i.e., 64 times smaller than the conductivity of steel, would be needed to keep Hvh,glob,out below 1 μm. Results are also interpreted based on contour plots of the axial and radial velocity variation of a retained analyte.
Originele taal2  English 

Artikelnummer  462452 
Aantal pagina's  12 
Tijdschrift  Journal of Chromatography A 
Volume  1654 
Nummer van het tijdschrift  2021 
DOI's  
Status  Published  8 aug 2021 
Bibliografische nota
Publisher Copyright:© 2021
Vingerafdruk
Duik in de onderzoeksthema's van 'Computational fluid dynamics study of potential solutions to alleviate viscous heating band broadening in 2.1 millimeter liquid chromatography columns'. Samen vormen ze een unieke vingerafdruk.Projecten
 1 Afgelopen

SRP6: SRP (Zwaartepunt): exploitatie van de voordelen van de Orde in Opsluiting voor een groenere chemie
Desmet, G., Denayer, J., Denayer, J., Desmet, G. & Denayer, J.
1/11/12 → 31/10/22
Project: Fundamenteel