Crystallization of two operator complexes from the Vibrio cholerae higBA2 toxin-antitoxin module

San Hadzi; Abel Garcia Pino; Kenn Gerdes; Jurij Lah; Remy Loris

doi:10.1107/S2053230X15000746

Crystallization of two operator complexes from the Vibrio cholerae higBA2 toxin-antitoxin module

San Hadzi, Abel Garcia Pino, Kenn Gerdes, Jurij Lah, Remy Loris

Toegepaste Biologische Wetenschappen

Onderzoeksoutput: Article › peer review

3 Citaten (Scopus)

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The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms for the HigA2 complexes and one crystal form for the HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221 with unit cell parameters a = b = 94.0 Å, c = 123.7 Å and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belongs space group P43212 and diffracts only to 3.45 Å. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belong to space group P41212 with a = b = 113.6 Å, c = 121.1 Å. They diffract to 3.3 Å resolution.

Originele taal-2	English
Pagina's (van-tot)	226-233
Aantal pagina's	8
Tijdschrift	Acta Crystallogr F Struct Biol Commun
Volume	71
DOI's	https://doi.org/10.1107/S2053230X15000746
Status	Published - 2015

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FWOAL756: Regulatiemechanismen van bacteriële toxine-antitoxine modules
Loris, R. (Administrative Promotor)
1/01/15 → 31/12/18
Project: Fundamenteel
FWOAL699: ESRF and DUBBLE: Synchrotron X-rays for revealing the structure and function of molecules and materials
Loris, R. (Administrative Promotor), Remaut, H. K. (Co-Promoter), Goderis, B. (Coördinator), Janssens, K. (Co-Promoter), Kirschhock, C. E. A. (Co-Promoter), Van Bael, M. (Co-Promoter), Poelman, D. (Co-Promoter), Temst, K. (Co-Promoter), Adriaens, A. (Co-Promoter) & Laszlo, V. (Co-Promoter)
1/01/13 → 31/12/17
Project: Fundamenteel
SRP13: Structuur en dynamiek van macromoleculaire complexen in gezondheid en ziekte
Steyaert, J. (Administrative Promotor), Remaut, H. K. (Co-Promoter), Loris, R. (Co-Promoter), Efremov, R. (Co-Promoter), Steyaert, J. (Administrative Promotor), Loris, R. (Co-Promoter) & Remaut, H. K. (Co-Promoter)
1/11/12 → 31/10/24
Project: Fundamenteel

3 Citaties
1 Article

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae
Hadzi, S., Zivic, Z., Kovacic, M., Zavrtanik, U., Haesaerts, S., Charlier, D., Plavec, J., Volkov, A. N., Lah, J. & Loris, R., 10 apr. 2024, In: Nat Commun. 15, 1, 12 blz., 3105.
Onderzoeksoutput: Article › peer review

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12 Citaten (Scopus)

29 Downloads (Pure)

2 Talk or presentation at a conference
1 Membership of external research organisation

6th FEMS Congress of european Microbiologists
Loris, R. (Invited speaker)
8 jun. 2015
Activiteit: Talk or presentation at a conference
6th FEMS Congress of European Microbiologists
Loris, R. (Invited speaker)
6 jun. 2015 → 11 jun. 2015
Activiteit: Talk or presentation at a conference
University of Southern Denmark (Externe Organisatie)
Loris, R. (Member)
1 jan. 2007 → …
Activiteit: Membership of external research organisation

Citeer dit

@article{a59f9da6417a40d18f2ab36b2f38aea5,

title = "Crystallization of two operator complexes from the Vibrio cholerae higBA2 toxin-antitoxin module",

abstract = "The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms for the HigA2 complexes and one crystal form for the HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221 with unit cell parameters a = b = 94.0 {\AA}, c = 123.7 {\AA} and diffract to 2.3 {\AA} resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belongs space group P43212 and diffracts only to 3.45 {\AA}. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belong to space group P41212 with a = b = 113.6 {\AA}, c = 121.1 {\AA}. They diffract to 3.3 {\AA} resolution.",

keywords = "Toxin-antitoxin module, Persistence, Persister, Protein-DNA complex, Protein-DNA interaction, Structural biology, Bacterial stress response, X-ray crystallography",

author = "San Hadzi and {Garcia Pino}, Abel and Kenn Gerdes and Jurij Lah and Remy Loris",

year = "2015",

doi = "10.1107/S2053230X15000746",

language = "English",

volume = "71",

pages = "226--233",

journal = "Acta Crystallogr F Struct Biol Commun",

issn = "1744-3091",

publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Crystallization of two operator complexes from the Vibrio cholerae higBA2 toxin-antitoxin module

AU - Hadzi, San

AU - Garcia Pino, Abel

AU - Gerdes, Kenn

AU - Lah, Jurij

AU - Loris, Remy

PY - 2015

Y1 - 2015

N2 - The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms for the HigA2 complexes and one crystal form for the HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221 with unit cell parameters a = b = 94.0 Å, c = 123.7 Å and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belongs space group P43212 and diffracts only to 3.45 Å. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belong to space group P41212 with a = b = 113.6 Å, c = 121.1 Å. They diffract to 3.3 Å resolution.

AB - The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms for the HigA2 complexes and one crystal form for the HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221 with unit cell parameters a = b = 94.0 Å, c = 123.7 Å and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belongs space group P43212 and diffracts only to 3.45 Å. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belong to space group P41212 with a = b = 113.6 Å, c = 121.1 Å. They diffract to 3.3 Å resolution.

KW - Toxin-antitoxin module

KW - Persistence

KW - Persister

KW - Protein-DNA complex

KW - Protein-DNA interaction

KW - Structural biology

KW - Bacterial stress response

KW - X-ray crystallography

U2 - 10.1107/S2053230X15000746

DO - 10.1107/S2053230X15000746

M3 - Article

SN - 1744-3091

VL - 71

SP - 226

EP - 233

JO - Acta Crystallogr F Struct Biol Commun

JF - Acta Crystallogr F Struct Biol Commun

ER -

Crystallization of two operator complexes from the Vibrio cholerae higBA2 toxin-antitoxin module

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Toegang tot document

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Vingerafdruk

Projecten

FWOAL756: Regulatiemechanismen van bacteriële toxine-antitoxine modules

FWOAL699: ESRF and DUBBLE: Synchrotron X-rays for revealing the structure and function of molecules and materials

SRP13: Structuur en dynamiek van macromoleculaire complexen in gezondheid en ziekte

Onderzoekersoutput

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae

Activiteiten

6th FEMS Congress of european Microbiologists

6th FEMS Congress of European Microbiologists

University of Southern Denmark (Externe Organisatie)

Citeer dit